Abstract: Chen Qianming*, Fang Jie, Wa Qingbiao, Yuan Wei, Wu Bo, Cai Qi, Lu Yonghong, Zhou Peimei, Jiang Cunhuo. *Department of Dermatology, Second People′s Hospital of Chengdu, Chengdu 610017, China Corresponding author: Yuan Wei, Email: dryuanweihua@sina.com 【Abstract】 Objective To investigate the mRNA expressions of PinX1 and hTERT in lesions of three skin tumors （including basal cell carcinoma （BCC）, squamous cell carcinoma （SCC） and actinic keratosis） as well as their significance. Methods This study included 36 tissue specimens from the lesions of 13 patients with BCC, 11 patients with SCC and 12 patients with actinic keratosis, as well as 15 tissue specimens from the normal skin of 15 individuals receiving surgical therapy. Real-time PCR was performed to quantify the mRNA expressions of PinX1 and hTERT in these specimens. Results The specimens from the lesions of the skin tumors showed a significantly lower expression of PinX1 mRNA （2.53 ± 1.57 vs. 4.25 ± 2.02, t = 3.273, P < 0.05）, but a higher expression of hTERT mRNA （6.37 ± 3.81 vs. 1.62 ± 0.99, t = 6.927, P < 0.05） compared with those from the normal skin. There was no significant correlation between the mRNA expressions of PinX1 and hTERT （P > 0.05）. Conclusion The down-regulated PinX1 expression and up-regulated hTERT expression may be associated with the activation and maintenance of telomerase in the formation of the three skin tumors.

Key words: Skin neoplasms, Genes, Pinx1, Genes, hTERT