中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (5): 309-312.

• 论著 • 上一篇    下一篇

氨基酮戊酸光动力治疗尖锐湿疣局部免疫反应的研究

张云凤1,缪飞2,王宏伟3,吕婷4,李晶晶5,王秀丽1   

  1. 1. 上海市皮肤病医院
    2. 复旦大学附属华东医院
    3. 复旦大学附属华东医院皮肤科
    4. 上海市复旦大学附属华东医院
    5. 苏州市第五人民医院皮肤科
  • 收稿日期:2012-06-19 修回日期:2012-07-17 出版日期:2013-05-15 发布日期:2013-05-01
  • 通讯作者: 王秀丽 E-mail:wangxiuli20150315@163.com

Local immune response in condyloma acuminatum treated with aminolevulinic acid-photodynamic therapy

yun-feng ZHANG1,Fei MIAOwang hong-wei 3, 3,   

  • Received:2012-06-19 Revised:2012-07-17 Online:2013-05-15 Published:2013-05-01

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摘要： 目的 探讨氨基酮戊酸光动力（ALA-PDT）治疗尖锐湿疣的局部免疫反应。方法 分别采用体外细胞共培养体系和在体免疫组化方法进行研究。将前期建立的稳定表达人乳头瘤病毒16E7蛋白HaCaT细胞株即HPV16E7/HaCaT细胞作为HPV感染角质形成细胞模型，将ALA-PDT处理后的HPV16E7/HaCaT细胞与外周血单一核细胞（PBMC）经Transwell小室共培养，3 h后观察PBMC的趋化情况；同时采用免疫组化方法研究10例尖锐湿疣患者光动力前及光动力后1、2、3、48 h时，皮损中CD4+ T细胞、CD8+ T细胞、CD68+巨噬细胞的数量及CD4+/CD8+T细胞数比值变化情况。结果 与ALA-PDT处理后的HPV16E7/HaCaT细胞共培养3 h后，PBMC有明显趋化现象。临床标本免疫组化结果显示，48 h 时CD4+T细胞、CD8+T细胞数量及其比值明显升高（P < 0.05），而CD68+巨噬细胞在3 h及48 h均升高（P < 0.05）。结论 ALA-PDT治疗尖锐湿疣的过程中，可诱导机体产生局部抗病毒免疫反应。 【关键词】 尖锐湿疣； 氨基酮戊酸； 光化学疗法； 免疫

关键词: 尖锐湿疣, 氨基酮戊酸, 光化学疗法, 免疫

Abstract: ZHANG Yun-feng*, MIU Fei, WANG Hong-wei, L?譈 Ting, LI Jing-jing, WANG Xiu-li. *Shanghai Skin Disease Hospital, Shanghai 200050, China Corresponding author: WANG Xiu-li, Email: xlwang2001@yahoo.cn 【Abstract】 Objective To investigate the local immune response in condyloma acuminatum treated with aminolevulinic acid-photodynamic therapy （ALA-PDT）. Methods In vitro and in vivo studies were performed. A previously established keratinocyte cell line human papilloma virus （HPV） 16E7/HaCaT which stably expresses HPV16E7 protein was used in this study. Peripheral blood mononuclear cells（PBMCs） were separated from 10 healthy volunteers. After pretreatment with ALA-PDT, HPV16E7/HaCaT cells were cocultured with the PBMCs for 3 hours in a Transwell chamber followed by the observation of chemotactic migration of PBMCs. Tissue samples were obtained from the lesions of 10 patients with condyloma acuminatum before, and at 1, 2, 3 and 48 hours after the first session of ALA-PDT. Immunohistochemistry was conducted to determine the number of CD4＋ T cells, CD8＋ T cells and CD68＋ macrophages as well as CD4/CD8 T-cell ratio in the tissue samples. Results After 3-hour coculture with HPV16E7/HaCaT cells pretreated by ALA-PDT, PBMCs showed apparent chemotactic migration. Immunohistochemistry revealed a statistical increase in the number of CD4＋ T cells , CD8＋ T cells and CD4/CD8 T-cell ratio at 48 hours （all P < 0.05），as well as in the number of CD68＋ macrophages at 3 hours and 48 hours （both P < 0.05） after the first session of ALA-PDT. Conclusion ALA-PDT may induce local antiviral immune response in condyloma acuminatum. 【Key words】 Condylomata acuminata; Aminolevulinic acid; Photochemotherapy; Immunity

Key words: Condyloma Acuminatum, aminolevulinic acid