中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (3): 172-175.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者CD4+ T细胞中miR-17和miR-20a 的表达及与病情活动的相关性研究

文韵诗1,秦海红2,徐金华3   

  1. 1. 复旦大学附属华山医院皮肤科
    2. 上海华山医院皮肤科
    3. 上海市复旦大学附属华山医院皮肤科
  • 收稿日期:2013-04-02 修回日期:2013-11-22 发布日期:2014-03-01
  • 通讯作者: 徐金华 E-mail:xjhhsyy@163.com,hsyyxjh@163.com
  • 基金资助:
    国家自然科学基金;上海市科委基础研究重点项目;上海市自然科学基金;上海市优秀学术带头人项目

Expressions of miR-17 and miR-20a in CD4+ T cells from patients with systemic lupus erythematosus and their correlations with disease activity

wen yunshi 2,XU Jinhua   

  • Received:2013-04-02 Revised:2013-11-22 Published:2014-03-01
  • Contact: XU Jinhua E-mail:xjhhsyy@163.com,hsyyxjh@163.com

PDF

7

摘要： 【摘要】 目的 探讨miR-17和miR-20a在系统性红斑狼疮（SLE）患者CD4+ T细胞中的表达及与疾病活动的相关性。 方法 应用实时荧光定量聚合酶链反应（PCR）检测SLE患者活动组17例、缓解组13例及健康对照组18例外周血CD4+ T细胞中miR-17和miR-20a的表达水平，并与SLE患者临床指标进行相关性分析。 结果 SLE患者组30例miR-17的表达水平（0.24 ± 0.08）显著高于健康对照组（0.15 ± 0.06），两组比较，P < 0.05；两组miR-20a的表达水平分别为0.19 ± 0.10和0.12 ± 0.09，两组比较，P < 0.05。SLE活动组、缓解组与健康对照组miR-17表达水平分别为0.27 ± 0.07、0.20 ± 0.05、0.16 ± 0.08，活动组高于缓解组（P < 0.05）和健康对照组（P < 0.05）；miR-20a表达水平三组分别为0.22 ± 0.10、0.15 ± 0.07、0.13 ± 0.09，活动组高于缓解组（P < 0.05）和健康对照组（P < 0.05）；缓解组miR-17和miR-20a的表达水平与健康对照组比较差异均无统计学意义（P > 0.05）。SLE患者组miR-17、miR-20a的表达与SLE活动指数（SLEDAI）均呈正相关（r = 0.45，P < 0.05和r = 0.38，P < 0.05），与抗dsDNA抗体滴度呈正相关（r = 0.54，P < 0.01和r = 0.46，P < 0.01），而与C3水平呈负相关（r = -0.43，P < 0.05和r = -0.42，P < 0.05）。 结论 SLE患者外周血CD4+ T细胞中miR-17、miR-20a表达上调，且miR-17、miR-20a表达水平与SLE病情活动相关。

关键词: 红斑狼疮，系统性, CD4阳性T淋巴细胞, miR-17, miR-20a

Abstract: Wen Yunshi, Qin Haihong, Xu Jinhua. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China Corresponding author: Xu Jinhua, Email: hsyyxjh@163.com 【Abstract】 Objective To investigate the expressions of miR-17 and miR-20a in CD4+ T cells from patients with systemic lupus erythematosus （SLE） and their correlations with disease activity. Methods CD4+ T cells were isolated from the venous blood of 30 patients with SLE and 18 healthy human controls. RNA was extracted from the CD4+ T cells, and real-time fluorescence-based quantitative PCR （qPCR） was performed to determine the expression levels of miR-17 and miR-20a. The intergroup comparison of miR-17 and miR-20a expression levels was done using t test and Mann-Whitney test, and the correlations of miR-17 and miR-20a expressions with clinical parameters were assessed using Pearson or Spearman correlation coefficients. Results The patients with SLE showed increased expression levels （2－△Ct） of miR-17 and miR-20a compared with the healthy controls （0.24 ± 0.08 vs. 0.15 ± 0.06 for miR-17, 0.19 ± 0.10 vs. 0.12 ± 0.09 for miR-20a, both P < 0.05）. The miR-17 and miR-20a expression levels were also significantly higher in patients with active SLE than in those with inactive SLE and the healthy controls （0.27 ± 0.07 vs. 0.20 ± 0.05 and 0.16 ± 0.08 for miR-17, 0.22 ± 0.10 vs. 0.15 ± 0.07 and 0.13 ± 0.09 for miR-20a, all P < 0.05）, but similar between the patients with inactive SLE and healthy controls （both P > 0.05）. The expression levels of both miR-17 and miR-20a were positively correlated with SLE disease activity index （SLEDAI） （r = 0.45, 0.38, respectively, both P < 0.05） and anti-dsDNA antibody level （r = 0.54, 0.46, respectively, both P < 0.05）, but negatively correlated with serum C3 levels （r = －0.43, －0.42, respectively, both P < 0.05）. Conclusions The expressions of miR-17 and miR-20a are increased in CD4+ T cells from patients with SLE, and correlated with the disease activity in SLE.

Key words: Lupus erythematosus, systemic, CD4-positive T-lymphocytes, miR-17, miR-20a

引用本文

文韵诗 秦海红 徐金华. 系统性红斑狼疮患者CD4+ T细胞中miR-17和miR-20a 的表达及与病情活动的相关性研究[J]. 中华皮肤科杂志, 2014,47(3):172-175. doi:

wen yunshi XU Jinhua. Expressions of miR-17 and miR-20a in CD4+ T cells from patients with systemic lupus erythematosus and their correlations with disease activity [J]. Chinese Journal of Dermatology, 2014, 47(3): 172-175.doi: