中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (2): 101-103.

• 论著 • 上一篇    下一篇

银屑病患者皮肤鳞屑真菌的分离及流式细胞计数分析

李艳佳1, 高顺强2, 林元珠2, 康彦考1, 邓秋敏1, 陈秀鉴3   

  1. 1. 石家庄市第一医院皮肤科 050011;
    2. 河北医科大学附属第四医院皮肤科;
    3. 河北医科大学病理教研室
  • 收稿日期:1998-02-19 修回日期:1998-07-17 出版日期:1999-04-15 发布日期:1999-04-15

Isolation and Flow Cytometry Analysis of Fungi in the Skin Scale of Psoriatic Patients

LIYanjia1, GAO Shunqiang2, LIN Yuanzhu2   

  1. Department of Dermatology, First Hospital of Shijiazhuang, Shijiazhuang 050011
  • Received:1998-02-19 Revised:1998-07-17 Online:1999-04-15 Published:1999-04-15

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摘要： 目的 了解真菌特别是糠秕孢子菌与银屑病的关系.方法 对82例银屑病患者和40例正常人的头皮、指甲、背部、上肢或下肢4个部位的皮肤鳞屑在3种不同培养基上进行了真菌培养分离,并应用流式细胞计数检测了8株银屑病皮损处糠秕孢子菌和4株正常人的糠秕孢子菌的DNA总含量及周期变化.结果 ①发现银屑病患者的真菌带菌率明显高于对照组(P<0.05),银屑病患者皮损处真菌阳性率与非皮损处的阳性率有非常显着性差异(P<0.01).②在银屑病组培养出59株糠秕孢子菌,而对照组仅有9株,有显着性差异.③S期的细胞比率和PI值银屑病组低于对照组.结论 真菌特别是糠秕孢子菌在银屑病患者的皮损处和正常皮肤上都较正常人高,对银屑病的发病有一定影响.

关键词: 银屑病, 真菌, 马拉色霉菌属

Abstract: Objective To investigate the relationship between fungi, especially Malassezia furfur and psoriasis. Methods The skin scale from scalps, nails, backs and extremities of 82 psoriatic patients and 40 healthy controls were collected and cultured on three media for isolation. Eight strains of M.furfur from psoriatic lesions and four strains from controls were analysed by flow cytometry. Results (1)It was found that the isolation rates of fungi were significantly higher in psoriatic group than those in controls (P<0.05). The isolation rates of the lesions were significantly higher than those of the uninvolved skin in psoriatic patients (P<0.01).(2)Fifty nine strains of M.furfur in psoriatic group and 9 strains in controls were cultured and the difference of isolation rate was significant. (3)The ratio of cells in S stage and proliferative index of isolated M.furfur were significantly lower in psoriatic group than those in controls. Conclusion The results indicate that fungi, especially M.furfur, are more frequently isolated in the lesions and uninvolved skin of psoriatic patients than those in healthy controls. It is suggested that fungi might play a role in the pathogenesis of psoriasis.

Key words: Psoriasis, Fungi, Malassezia