中华皮肤科杂志 ›› 2023, Vol. 56 ›› Issue (7): 651-656.doi: 10.35541/cjd.20220655

• 论著 • 上一篇    下一篇

特应性皮炎患者外周血单个核细胞转谷氨酰胺酶2的表达与相关炎症因子及疾病严重程度的相关性

渠莉田1，2    陈锦丛1    程波2    苏惠春1，2   

  1. 1福建医科大学附属协和医院皮肤科，福州  350001；2福建医科大学附属第一医院皮肤科，福州  350004
  • 收稿日期:2022-09-13 修回日期:2023-05-08 发布日期:2023-07-04
  • 通讯作者: 程波；苏惠春 E-mail:suhuichun@163.com
  • 作者简介:chengbo630415@126.com; suhuichun@163.com
  • 基金资助:
    福建省自然科学基金（2019J01310）；福建医科大学附属协和医院引进人才启动基金（2021XH026）

Correlations of transglutaminase 2 expression in peripheral blood mononuclear cells with relevant inflammatory factors and disease severity in patients with atopic dermatitis

Qu Litian1,2, Chen Jincong1, Cheng Bo2, Su Huichun1,2   

  1. 1Department of Dermatology, Fujian Medical University Union Hospital, Fuzhou 350001, China; 2Department of Dermatology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, China
  • Received:2022-09-13 Revised:2023-05-08 Published:2023-07-04
  • Contact: Cheng Bo; Su Huichun E-mail:suhuichun@163.com
  • Supported by:
    Natural Science Foundation of Fujian Province of China （2019J01310）; The Investigator Initiation Fund Project of Fujian Medical University Union Hospital （2021XH026）

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摘要： 【摘要】 目的 分析特应性皮炎（AD）患者外周血单个核细胞（PBMC）中转谷氨酰胺酶2（TGM2）的表达及其与AD相关炎症因子和疾病严重程度的相关性。方法 收集福建医科大学附属第一医院2020年7月至2021年1月皮肤科门诊及住院治疗的29例AD患者及15例健康对照。采集受试者10 ml外周血及临床相关信息［包括年龄、性别、病程、血常规（包括嗜酸性粒细胞、嗜碱性粒细胞计数）、总IgE水平、AD评分（SCORAD）等］。用密度梯度离心法分离PBMC后，通过荧光定量PCR与流式细胞仪检测29例AD患者及15例健康对照PBMC中TGM2 mRNA和PBMC表面TGM2蛋白的表达，及PBMC中AD相关炎症因子白细胞介素（IL）1β、IL-4、IL-6、IL-8、IL-10、IL-13、IL-17、胸腺基质淋巴细胞生成素（TSLP）、P2RX7（嘌呤能受体P2X，配体门控离子通道7）等的mRNA表达。采用Mann-Whitney U检验分析组间差异，相关性评估采用Spearman相关性分析。结果 AD患者PBMC中TGM2 mRNA相对表达水平与对照组差异无统计学意义［M（Q1，Q3）：0.509（0.325，0.958）比0.475（0.328，1.051），U = 210.50，P = 0.872］，AD组IL-4 mRNA表达水平低于对照组［0.171（0.049，0.449）比0.824（0.397，1.378），P < 0.001］，IL-8、IL-13 mRNA表达水平均高于对照组（P = 0.011、0.006）。Spearman相关性分析显示，AD患者组PBMC中TGM2 mRNA表达水平与炎症因子IL-4和P2RX7 mRNA的表达正相关（rs = 0.42、0.40，P = 0.024、0.034）；与AD患者疾病严重程度相关指标如年龄［21（16，29）岁］、病程［4（1，10）年］、嗜酸性粒细胞计数［0.33（0.18，0.65） × 109/L］、嗜碱性粒细胞计数［0.04（0.03，0.06） × 109/L］、SCORAD评分［60.5（46.98，66.13）］、血清总IgE水平［373（40，1815） IU/ml］均无显著相关性（均P > 0.05）。AD组与对照组PBMC表面TGM2蛋白的相对表达水平［54.9（47.6，62.8）比55.55（51.5，60.25）］差异无统计学意义（U = 112.00，P = 0.922），AD患者PBMC表面TGM2蛋白的表达水平与疾病严重程度相关指标均无显著相关性（P＞0.05）。结论 AD患者PBMC中TGM2 mRNA及PBMC表面TGM2蛋白的表达与健康对照间无明显差异，且与AD疾病严重程度无相关性。

关键词: 皮炎, 特应性, 转谷酰胺酶, 外周血单个核细胞, 白细胞介素4, 白细胞介素8, 白细胞介素13, 嘌呤能受体P2X, 配体门控离子通道7

Abstract: 【Abstract】 Objective To determine the expression of transglutaminase 2 （TGM2） in peripheral blood mononuclear cells （PBMCs） from patients with atopic dermatitis （AD）, and to analyze its correlation with AD-related inflammatory factors and disease severity. Methods A total of 29 AD patients and 15 healthy controls were collected from the First Affiliated Hospital of Fujian Medical University from July 2020 to January 2021. Ten milliliters of peripheral blood samples were collected from each subject, so was the clinical information, including age, gender, course of disease, eosinophil counts, basophil counts, total IgE levels, Scoring AD index （SCORAD）, etc. PBMCs were isolated by density gradient centrifugation. Fluorescence-based quantitative PCR was performed to determine the mRNA expression of TGM2 and AD-related inflammatory factors （interleukin ［IL］-1β, IL-4, IL-6, IL-8, IL-10, IL-13, IL-17, thymic stromal lymphopoietin ［TSLP］, P2RX7 ［purinergic receptor P2X, ligand-gated ion channel, 7］, etc.） in PBMCs from 29 AD patients and 15 healthy controls, and flow cytometry to determine TGM2 protein expression on PBMCs. Mann-Whitney U test was used to analyze differences between groups, and Spearman correlation analysis to evaluate the correlation. Results The relative mRNA expression of TGM2 in PBMCs did not differ between the AD group and control group （M［Q1, Q3］: 0.509 ［0.325, 0.958］ vs. 0.475 ［0.328, 1.051］, U = 210.50, P = 0.872）. Compared with the control group, the AD group showed significantly decreased IL-4 mRNA expression （0.171［0.049, 0.449］ vs. 0.824 ［0.397, 1.378］, P < 0.001）, but significantly increased mRNA expression of IL-8 and IL-13 （P = 0.011, 0.006, respectively）. Spearman correlation analysis showed that the mRNA expression level of TGM2 in PBMCs was positively correlated with the mRNA expression levels of IL-4 and P2RX7 in the AD group （rs = 0.42, 0.40, P = 0.024, 0.034, respectively）, while there were no correlations between TGM2 mRNA expression and AD severity-related indicators （all P＞0.05）, such as age （21 ［16, 29］ years）, course of disease （4［1,10］ years）, eosinophil counts （0.33［0.18, 0.65］ × 109/L）, basophil counts （0.04 ［0.03, 0.06］ × 109/L］, SCORAD scores （60.5 ［46.98, 66.13］ points）, and serum total IgE levels （373 ［40, 1 815］ IU/ml）. The relative protein expression levels of TGM2 on the surface of PBMCs did not differ between the AD group and control group （54.9 ［47.6, 62.8］ vs. 55.55 ［51.5, 60.25］, U = 112.00, P = 0.922）］, and no correlations were observed between the protein expression of TGM2 on PBMCs and AD severity-related indicators in the AD group（all P ＞ 0.05）. Conclusion No significant differences were observed in TGM2 mRNA expression in PBMCs or TGM2 protein expression on the surface of PBMCs between the AD patients and healthy controls, and there were no correlations between the TGM2 mRNA and protein expression and AD severity.

Key words: Dermatitis, atopic, Transglutaminases, Peripheral blood mononuclear cell, Interleukin-4, Interleukin-8, Interleukin-13, Purinergic receptor P2X, ligand-gated ion channel, 7

引用本文

渠莉田 陈锦丛 程波 苏惠春. 特应性皮炎患者外周血单个核细胞转谷氨酰胺酶2的表达与相关炎症因子及疾病严重程度的相关性[J]. 中华皮肤科杂志, 2023,56(7):651-656. doi:10.35541/cjd.20220655

Qu Litian, Chen Jincong, Cheng Bo, Su Huichun, . Correlations of transglutaminase 2 expression in peripheral blood mononuclear cells with relevant inflammatory factors and disease severity in patients with atopic dermatitis[J]. Chinese Journal of Dermatology, 2023, 56(7): 651-656.doi:10.35541/cjd.20220655