Abstract:

Cao Lei, Cheng Hao*, Wang Hui, Zhou Qiang, Tang Yi, Jiang Shaojie, Ding Yang, Chen Xianzhen. *Department of Dermatology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China Corresponding author: Cheng Hao, Email: hz_chenghao@126.com 【Abstract】 Objective To detect the expressions of human papillomavirus （HPV）-6b/11 E7 proteins in condyloma acuminatum lesions in external genitalia with home-made polyclonal antibodies against HPV-6b/11 E7 proteins, and to estimate the clinical application value of the polyclonal antibodies. Methods Fifty-five tissue specimens were collected from the lesions of patients with clinically and pathologically comfirmed condyloma acuminatum, and subjected to paraffin embedding. An immunohistochemical study was conducted to detect the expressions of HPV-6b/11 E7 proteins in the 55 specimens with home-made polyclonal antibodies against HPV-6b/11 E7 proteins. Reverse transcription （RT）-PCR was performed to quantify the mRNA expressions of HPV-6b/11 E7 in 18 frozen specimens from these patients. The coincidence rate between the two methods was analyzed. Results Immunohistochemical staining showed that HPV-6b/11 E7 proteins were expressed in the nuclei of cells throughout the whole epidermis of the lesions, and especially in the basal layer. Of the 55 specimens, 42 （76.36%） stained positive for the HPV-6b E7 protein, 32 （58.18%） for the HPV11 E7 protein, 22 （40.00%） for both of them, 3 （5.45%） for neither of them, with the positive rate of E7 proteins being 94.55% （52/55）. As RT-PCR showed, the HPV-6b and HPV 11 E7 mRNAs were positive in 15 and 10 out of the 18 frozen specimens, respectively, and both positive in 7 specimens. The HPV types identified by RT-PCR in the 15 specimens were fully consistent with those by the immunohistochemical study. Conclusion The immunohistochemical assay using the home-made anti-HPV-6b/11 E7 polyclonal antibodies can be performed to detect condyloma acuminatum lesions and to observe the distribution of HPV-6b/11-infected cells in an intuitionistic way.