中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (11): 775-778.

• 论著 • 上一篇    下一篇

聚合酶链反应Array法检测寻常性银屑病皮损核因子κB相关信号通路

胡燕荣1,刘建勇2,张德志3,普雄明4   

  1. 1. 新疆维吾尔自治区人民医院高血压研究所
    2. 新疆维吾尔自治区人民医院皮肤性病科
    3. 新疆维吾尔自治区人民医院
    4. 乌鲁木齐市新疆维吾尔自治区人民医院皮肤科
  • 收稿日期:2012-11-26 修回日期:2013-06-27 出版日期:2013-11-15 发布日期:2013-11-01
  • 通讯作者: 普雄明 E-mail:puxiongming@126.com
  • 基金资助:
    核转录因子NF-κB与新疆地区维吾尔族银屑病相关性研究

Detection of signaling pathways related to nuclear factor-κB in patients with psoriasis vulgaris using PCR-array analysis

Yan-rong1,Jian-Yong LIU2,Zhang DeZhi   

  • Received:2012-11-26 Revised:2013-06-27 Online:2013-11-15 Published:2013-11-01

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摘要： 【摘要】 目的 检测维吾尔族银屑病患者皮损组织与核因子κB（NF-κB）相关的84个信号分子的表达，初步探讨表达明显异常的信号分子。 方法 收集8例新疆维吾尔族银屑病患者皮损组织，同时收集患者的正常皮肤组织作为对照，提取组织总RNA，逆转录生成cDNA后，聚合酶链反应Array法（PCR-Array）检测NF-κB相关的84个信号分子表达水平，以2倍为上调或者下调的检验标准。 结果 84种信号分子中，22种信号分子表达上调，7种下调。其中，上调最为明显的分子为胱冬肽酶募集结构域（CARD11）及粒细胞巨噬细胞集落刺激因子（CSF2），下调最为明显的为肿瘤坏死因子超家族成员5（CD40）、白介素10（IL-10）及B细胞κ轻肽基因增强子核因子抑制因子ε（NFκBIE）。 结论 在检测的银屑病患者皮损组织中，NF-κB信号通路相关的多种分子可能处于被激活状态或抑制状态。 【关键词】 银屑病； 寡核苷酸序列分析； NF-κB； 粒细胞巨噬细胞集落刺激因子； CARD11

关键词: 银屑病, 粒细胞巨噬细胞集落刺激因子, NF-κB, CARD11, 寡核苷酸序列分析

Abstract: HU Yan-rong, LIU Jian-yong, ZHANG De-zhi, PU Xiong-ming. Department of Dermatology, People′s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China Corresponding author: PU Xiong-ming, Email: puxiongming@126.com 【Abstract】 Objective To detect the expression of 84 signaling molecules associated with nuclear factor-κB in lesions of Uygur patients with psoriasis. Methods Skin specimens were obtained from the lesional and paralesional skin of eight Uygur patients with psoriasis. Total RNA was extracted from the skin specimens and reversely transcribed into cDNA. PCR-array analysis was carried out to quantify the expressions of 84 signaling molecules related to nuclear factor-κB. Genes with a fold-change > or = 2 were defined as differentially expressed. Results Among the 84 tested genes, 22 showed upregulated expression, 7 downregulated expression, and the remaining 54 genes showed no significant changes in psoriatic lesions compared with the normal skin. The strongest upregulation was observed in the gene expressions of Caspase recruitment domain family 11 （CARD11） and granulocyte-macrophage colony-stimulating factor 2 （CSF2）, and the most significant downregulation in the gene expression of interleukin 10 （IL-10）, tumor necrosis factor superfamily member 5 （CD40） and nuclear factor of kappa light polypeptide gene enhancer in B-cell inhibitor, epsilon（NFκBIE）. Conclusion Multiple molecules involved in the NF-κB signaling pathway might be activated or inhibited in lesions of patients with psoriasis. 【Key words】 Psoriasis; Oligonucleotide array sequence analysis; NF-kappaB; Granulocyte-macrophage colony-stimulating factor; CARD11

Key words: NF-κB, CARD11

中图分类号: 

  • R751