中华皮肤科杂志 ›› 2015, Vol. 48 ›› Issue (7): 459-462.

• 论著 • 上一篇    下一篇

整合素在瘢痕疙瘩间质干细胞中的表达研究

宋海峰1,王文婷2,刘涛1,张衍国2   

  1. 1. 第四军医大学唐都医院
    2. 第四军医大学唐都医院皮肤科
  • 收稿日期:2014-09-16 修回日期:2014-12-06 发布日期:2015-06-30
  • 通讯作者: 刘涛 E-mail:mengxh@fmmu.edu.cn
  • 基金资助:

    前列腺酸性磷酸酶与吗啡的协同镇痛机制

Expressions of integrin in human keloid-derived mesenchymal stem cells

  • Received:2014-09-16 Revised:2014-12-06 Published:2015-06-30
  • Contact: Tao LIU E-mail:mengxh@fmmu.edu.cn

摘要:

目的 了解瘢痕疙瘩间质干细胞中整合素9种亚基mRNA和蛋白的表达。 方法 以培养的瘢痕疙瘩间质干细胞为研究对象,以正常皮肤的间质干细胞为对照,运用实时定量PCR和Western印迹法分别检测其mRNA和蛋白的表达情况。 结果 实时定量PCR检测结果表明,瘢痕疙瘩间质干细胞中整合素α2、α3、α5、αV、α10、α11、β1 mRNA的表达水平与正常皮肤干细胞相比,差异无统计学意义(P > 0.05),α8 mRNA的表达水平显著低于正常皮肤干细胞,而β3 mRNA的表达水平则显著增高(均P < 0.01)。Western印迹检测表明,两种干细胞α8和β3蛋白表达水平变化与其mRNA变化一致(P < 0.01)。 结论 整合素α8和β3可能与瘢痕疙瘩的发生发展有关,其组成的相应受体可能与瘢痕疙瘩的发病机制相关。

关键词: 整合素&alpha, 8

Abstract:

Song Haifeng, Wang Wenting, Liu Tao, Zhang Yanguo. Department of Dermatology, Tangdu Hospital, Fourth Military Medical University, Xi′an 710038, China Corresponding authors: Liu Tao, Email: Morlab2@fmmu.edu.cn; Zhang Yanguo, Email: zhangya3341@163.com 【Abstract】 Objective To investigate the mRNA and protein expressions of nine integrin subunits in human keloid-derived mesenchymal stem cells (KD-MSCs). Methods Cultured KD-MSCs and normal skin-derived MSCs (NS-MSCs) served as the experiment group and control group respectively. Real-time quantitative PCR and Western blot were performed to measure the mRNA and protein expressions of nine integrin subunits in the two groups respectively. Statistical analysis was carried out by t test. Results Real-time quantitative PCR showed no significant difference in the mRNA expression level of any of the integrin units α2, α3, α5, αV, α10, α11 or β1 between KD-MSCs and NS-MSCs (all P > 0.05). The mRNA expression level of integrin α8 was decreased, while that of integrin β3 was significantly increased in KD-MSCs compared with NS-MSCs (both P < 0.01). Western blot revealed that the changes in protein expression levels of integrin units α8 and β3 were consistent with those in their mRNA expression levels in both KD-MSCs and NS-MSCs (both P < 0.01). Conclusions Integrin units α8 and β3 may be involved in the occurrence and development of keloid, and the receptors made up of them may play important roles in the pathogenesis of keloid.

引用本文

宋海峰 王文婷 刘涛 张衍国. 整合素在瘢痕疙瘩间质干细胞中的表达研究[J]. 中华皮肤科杂志, 2015,48(7):459-462. doi: