中华皮肤科杂志 ›› 2023, Vol. 56 ›› Issue (3): 234-240.doi: 10.35541/cjd.20220542

• 论著 • 上一篇    下一篇

带状疱疹患者外周血CD100表达变化及其对CD8+ T细胞功能的调控作用

郑晶冰1    韩会敏1    王珊2    孙倩1   

  1. 1驻马店市中心医院医学检验科，驻马店  463000；2郑州大学附属儿童医院/河南省儿童医院/郑州儿童医院检验科，郑州  450000
  • 收稿日期:2022-08-02 修回日期:2022-12-21 发布日期:2023-03-06
  • 通讯作者: 郑晶冰 E-mail:790035736@qq.com
  • 基金资助:
    河南省医学科技攻关计划（联合共建）项目（LHGJ20190413）

Changes in CD100 expression and its regulation on CD8+ T cell function in peripheral blood of patients with herpes zoster

Zheng Jingbing1, Han Huimin1, Wang Shan2, Sun Qian1   

  1. 1Department of Clinical Laboratory, Zhumadian Central Hospital, Zhumadian 463000, Henan, China; 2Department of Clinical Laboratory, Children′s Hospital Affiliated of Zhengzhou University/Henan Children′s Hospital/Zhengzhou Children′s Hospital, Zhengzhou 450000, China
  • Received:2022-08-02 Revised:2022-12-21 Published:2023-03-06
  • Contact: Zheng Jingbing E-mail:790035736@qq.com
  • Supported by:
    Joint Construction Project of Medical Science and Technology Research Program of Henan Province（LHGJ20190413）

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摘要： 【摘要】 目的 检测带状疱疹患者血浆可溶型CD100（sCD100）和外周血T细胞中膜型CD100（mCD100）表达变化，观察外源性CD100对带状疱疹患者CD8+ T细胞的功能调控。方法 收集2019年7月至2021年4月在驻马店市中心医院就诊的带状疱疹患者53例以及入组年龄和性别匹配的健康对照25例。采集受试者静脉抗凝血，分离血浆和外周血单个核细胞，酶联免疫吸附试验检测血浆sCD100水平，流式细胞仪检测CD4+ T细胞和CD8+ T细胞中mCD100表达。纯化CD8+ T细胞，比较带状疱疹患者和对照CD8+ T细胞分泌毒性分子和细胞因子的差异。使用重组人CD100和重组水痘-带状疱疹病毒糖蛋白刺激带状疱疹患者纯化的CD8+ T细胞，观察重组人CD100对CD8+ T细胞分泌毒性分子和细胞因子的影响。组间比较采用t检验。结果 带状疱疹组血浆sCD100水平［（1.12 ± 0.23） ng/ml］低于对照组［（1.31 ± 0.28） ng/ml，t = 2.97，P = 0.004］，mCD100+CD8+ T细胞比例（17.41% ± 4.26%）高于对照组（14.69% ± 3.70%，t = 2.52，P = 0.014），两组间mCD100+CD4+ T细胞比例差异无统计学意义（2.52% ± 0.58%比2.32% ± 0.56%，t = 1.27，P = 0.208）。带状疱疹患者CD8+ T细胞中穿孔素、颗粒酶B mRNA表达以及穿孔素、颗粒酶B、γ干扰素、肿瘤坏死因子α分泌水平均低于对照组（P < 0.05）。重组人CD100刺激后，CD8+ T细胞培养上清液中穿孔素、颗粒酶B、IFN-γ、TNF-α水平［（43.68 ± 14.12）、（126.8 ± 22.92）、（12.79 ± 3.66）、（310.0 ± 79.90） pg/ml］均高于无CD100刺激组［（34.55 ± 10.78）、（99.04 ± 10.44）、（9.53 ± 2.00）、（275.6 ± 68.04） pg/ml，均P < 0.05］。结论 带状疱疹患者存在sCD100和mCD100表达失衡，外源性sCD100可增强带状疱疹患者CD8+ T细胞的杀伤功能。

关键词: 带状疱疹, CD8阳性T淋巴细胞, CD100, 免疫调控

Abstract: 【Abstract】 Objective To investigate changes in expression of plasma soluble CD100 （sCD100） and membrane-bound CD100 （mCD100） on peripheral T cells in patients with herpes zoster, and to observe the regulatory function of exogenous CD100 on CD8+ T cells. Methods A total of 53 patients with herpes zoster attending the Zhumadian Central Hospital from July 2019 to April 2021 were enrolled, so were 25 age- and sex-matched healthy controls. Anticoagulated venous blood samples were collected, plasma and peripheral blood mononuclear cells were isolated, plasma sCD100 levels were detected by enzyme-linked immunosorbent assay, and mCD100 expression on CD4+ and CD8+ T cells was determined by flow cytometry. After the purification of CD8+ T cells, the secretion levels of cytotoxic molecules and cytokines by CD8+ T cells were measured and compared between herpes zoster patients and controls. Some purified CD8+ T cells from herpes zoster patients were stimulated with recombinant human CD100 and recombinant varicella-zoster virus glycoprotein, and the effect of recombinant human CD100 on the secretion of cytotoxic molecules and cytokines by CD8+ T cells was investigated. Comparisons between groups were conducted by t test. Results Plasma sCD100 levels were significantly lower in the herpes zoster group （1.12 ± 0.23 ng/ml） than in the control group （1.31 ± 0.28 ng/ml, t = 2.97, P = 0.004）, the proportion of mCD100 + CD8+ T cells was significantly higher in the herpes zoster group （17.41% ± 4.26%） than in the control group （14.69% ± 3.70%, t = 2.52, P = 0.014）, and no significant difference in the proportion of mCD100 + CD4+ T cells was found between the two groups （2.52% ± 0.58% vs. 2.32% ± 0.56%, t = 1.27, P = 0.208）. The herpes zoster group showed significantly decreased mRNA expression of perforin and granzyme B in, and lower secretion levels of perforin, granzyme B, interferon-γ and tumor necrosis factor-α by CD8+ T cells compared with the control group （all P < 0.05）. After stimulation with recombinant human CD100, levels of perforin, granzyme B, interferon-γ and tumor necrosis factor-α in the culture supernatant of CD8+ T cells （43.68 ± 14.12, 126.8 ± 22.92, 12.79 ± 3.66, 310.0 ± 79.90 pg/ml, respectively ） were significantly higher than those in non-stimulated group （34.55 ± 10.78, 99.04 ± 10.44, 9.53 ± 2.00, 275.6 ± 68.04 pg/ml, respectively, all P < 0.05）. Conclusion There was an imbalance between sCD100 and mCD100 expression in patients with herpes zoster, and exogenous sCD100 may enhance the cytotoxicity of CD8+ T cells in herpes zoster patients.

Key words: Herpes Zoster, CD8-positive T-lymphocytes, CD100, Immunoregulation

引用本文

郑晶冰 韩会敏 王珊 孙倩. 带状疱疹患者外周血CD100表达变化及其对CD8+ T细胞功能的调控作用[J]. 中华皮肤科杂志, 2023,56(3):234-240. doi:10.35541/cjd.20220542

Zheng Jingbing, Han Huimin, Wang Shan, Sun Qian. Changes in CD100 expression and its regulation on CD8+ T cell function in peripheral blood of patients with herpes zoster[J]. Chinese Journal of Dermatology, 2023, 56(3): 234-240.doi:10.35541/cjd.20220542