中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (8): 572-574.

• 论著 • 上一篇    下一篇

培养的人皮肤成纤维细胞光老化模型中自噬水平的研究

张青松1,鞠梅2,陈崑2,李新宇2,常宝珠2,顾恒3   

  1. 1. 常熟 常熟市新区医院
    2. 南京 中国医学科学院北京协和医学院皮肤病研究所
    3. 中国医学科学院皮肤病医院(研究所)理疗科
  • 收稿日期:2009-11-19 修回日期:2010-03-31 出版日期:2010-08-15 发布日期:2010-08-10
  • 通讯作者: 张青松 E-mail:49lzy@163.com
  • 基金资助:

    江苏省自然科学基金

Autophagy in human skin fibroblast model for photoaging

1, 1, 1, 1, 1   

  • Received:2009-11-19 Revised:2010-03-31 Online:2010-08-15 Published:2010-08-10

摘要:

目的 研究人皮肤成纤维细胞的光老化模型中细胞自噬功能的变化。方法 通过UVB多次照射培养的人皮肤成纤维细胞,制作光老化人皮肤成纤维细胞模型,同时设立不用UVB照射的对照组。用β-半乳糖苷酶法测定未照射的对照组及光老化组细胞的老化程度,并分别测定两组细胞的自噬体表达水平。结果 经UVB重复照射后的光老化人皮肤成纤维细胞大多变形、扭曲,部分细胞死亡。光老化组与对照组β-半乳糖苷酶表达阳性细胞的百分率分别为50.60% ± 5.04%、14.58% ± 2.69%,两组间差异有统计学意义(P < 0.01)。光老化组与正常对照组自噬体阳性细胞百分率分别为14.91% ± 4.59%、68.45% ± 8.25%,两组间差异有统计学意义(P < 0.01)。结论 人皮肤成纤维细胞光老化模型形态明显异常,生长基本停滞,老化程度明显高于正常细胞,而自噬水平则明显低于正常对照组细胞。

关键词: 自噬, 光老化, 成纤维细胞

Abstract:

Objective To observe the changes of autophagy in human skin fibroblast (HSF) model for photoaging. Methods HSF model for photoaging was established through repeated exposure to ultraviolet B (UVB). Those HSFs receiving no irradiation served as the control. The degree of aging was evaluated by β-galactosidase assay, and autophagy level was detected. Results After repeated exposure to UVB, most photoaged HSFs were deformed and distorted, and some of them even died. The percentage of β-galactosidase-positive cells was 50.60% ± 5.04% and 14.58% ± 2.69%, respectively in photoaged and control HSFs (P < 0.01). Significant difference was also observed in the proportion of cytophagosome-positive cells between photoaged and control HSFs (14.91% ± 4.59% vs 68.45% ± 8.25%, P < 0.01). Conclusion The HSF model for photoaging shows obviously abnormal appearance and stagnant growth with increased degree of senescence and decreased autophagy compared with normal control HSFs.

Key words: autophagy, photoaging, fibroblast