中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (7): 474-477.

• 论著 • 上一篇    下一篇

桥粒芯糖蛋白3刺激下寻常型天疱疮患者外周血T淋巴细胞增殖检测分析

胡婕1,魏仲香2,姜奕3,梁再赋4,耿龙3   

  1. 1. 中国医科大学附属第一医院皮肤病与性病科
    2.
    3. 沈阳 中国医科大学附属第一医院皮肤科
    4. 沈阳 中国医科大学实验技术中心一部
  • 收稿日期:2009-07-02 修回日期:2010-02-26 出版日期:2010-07-15 发布日期:2010-07-13
  • 通讯作者: 胡婕 E-mail:hujie_cmu_006204@yahoo.cn
  • 基金资助:

    教育部创新团队支持计划项目;国家自然基金

Proliferation of desmoglein 3-stimulated T lymphocytes from patients with pemphigus vulgaris

  • Received:2009-07-02 Revised:2010-02-26 Online:2010-07-15 Published:2010-07-13
  • Contact: HU Jie E-mail:hujie_cmu_006204@yahoo.cn

摘要:

目的 观察桥粒芯糖蛋白3(Dsg3)对寻常型天疱疮(PV)患者外周血T淋巴细胞增殖的影响。方法 PV患者12例,正常人22例,提取外周血单一核细胞(PBMC),采用流式细胞仪技术分别检测Dsg3刺激下PBMC中T淋巴细胞亚群变化及T淋巴细胞增殖情况,分析PV患者与正常人之间存在的差异。结果 PV患者PBMC在Dsg3刺激下Th2型T淋巴细胞所占比例为12.17% ± 5.32%,Th1型T淋巴细胞所占比例为4.08% ± 1.50%;未经Dsg3刺激的阴性对照组分别为9.84% ± 5.41%和3.91% ± 1.38%。PV患者PBMC在Dsg3刺激和无Dsg3刺激时的Th2型T淋巴细胞所占比例均显著高于正常人(P < 0.05);Dsg3刺激下PV患者T淋巴细胞发生增殖,CD4+ T淋巴细胞增殖率为4.65% ± 3.28%,显著高于正常人(P < 0.05)。结论 Dsg3可诱导PV患者CD4+ T淋巴细胞发生特异性增殖,且主要为Th2优势型。

关键词: 寻常型天疱疮, desmoglein 3, T淋巴细胞, Th1/Th2, 流式细胞仪

Abstract:

Objective To investigate the impact of desmoglein 3 (Dsg3) on the proliferation of peripheral T lymphocytes from patients with pemphigus vulgaris (PV). Methods Peripheral blood mononuclear cells (PBMCs) were obtained from 12 patients with PV and 22 normal human controls, cultured with or without the presence of Dsg3 or phytohemagglutinin for 3 days. Flow cytometry was performed to detect the changes of T-lymphocyte subsets in PBMCs stimulated with Dsg3 and proliferation of T-lymphocytes. Results In patients with PV, the percentage of Th2 and Th1 cells was 12.17% ± 5.32% and 4.08% ± 1.50%, respectively in Dsg3-stimulated PBMCs, 9.84% ± 5.41% and 3.91% ± 1.38%, respectively in non-stimulated PBMCs. Increased percentage of Th2 cells was observed in Dsg3-stimulated and non-stimulated PBMCs from patients with PV compared with those from normal human controls (both P < 0.05). After stimulation with Dsg3, there was a significant proliferation of T cells from patients, and the proliferation rate of CD4+ T cells was 4.65% ± 3.28%, which was significantly higher than that from normal controls (P < 0.05). Conclusion Dsg3 can induce the specific proliferation of CD4+ T cells, especially Th2 type CD4+ T cells, from patients with PV.

Key words: Pemphigus vulgaris, desmoglein 3, T lymphocytes, Th1/Th2, Flow cytometry