中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (10): 731-732.

• 短篇论著 • 上一篇    下一篇

白癜风患者病程与抗酪氨酸酶抗体的相关分析

周妙妮1,关翠萍2,洪为松2,许爱娥3   

  1. 1. 杭州市第三人民医院皮肤科
    2. 杭州市第三人民医院
    3. 安徽医科大学附属杭州市第三人民医院皮肤科
  • 收稿日期:2010-02-25 修回日期:2010-06-23 出版日期:2010-10-15 发布日期:2010-10-09
  • 通讯作者: 许爱娥 E-mail:xuaiehz@msn.com
  • 基金资助:

    白癜风患者TYR相关蛋白抗体检测试剂盒研制及应用;白癜风患者抗黑素细胞抗体检测靶抗原筛选及临床诊断研究

Association analysis between disease course and anti-tyrosinase antibody levels in patients with vitiligo

  • Received:2010-02-25 Revised:2010-06-23 Online:2010-10-15 Published:2010-10-09

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摘要：

目的 应用酪氨酸酶表达肽TYR240-479作为抗原，检测654例白癜风患者血清抗酪氨酸酶IgG抗体水平，分析白癜风患者病程与体内抗酪氨酸酶抗体水平的关联性。方法 以TYR240-479作为包被抗原，应用ELISA法检测白癜风患者血清中抗酪氨酸酶IgG抗体的滴度。不同病程患者抗体阳性检出率的差异以及病程与抗体水平的关联性分析应用卡方检验，多组率的两两比较应用Bonferroni校正。平均滴度差异比较应用one-way ANOVA，组间两两比较应用LSD－t检验。结果 各病程组抗体检测总阳性率具有显著性差异，P＝0.027，r＝0.112。其中病程1～5年（含5年）组与5～10年（含10年）组具有显著性差异，P＝0.007。病程是否超过5年与抗体水平的关联强度分析表明病程与抗体水平有关联，P＝0.025，OR＝1.473，95％CI（1.049-2.068），但各病程组间抗体平均滴度无显著性差异。 结论 与病程超过5年的患者相比，病程5年之内的患者抗体阳性率更高，与抗体水平的关联更为紧密。白癜风病程与患者抗酪氨酸酶抗体水平有一定的关联性。

关键词: 白癜风, 病程, 一元酚单氧酶, IgG抗体, 关联分析

Abstract:

Objective To detect IgG antibody against human tyrosinase in vitiligo patients using expression peptide TYR240-479, and analyze the association between the antibody level and the duration in vitiligo patients. Methods TYR240-479 as antigen, ELISA was carried out to detect the titer of IgG antibody against human tyrosinase in vitiligo patients. The difference of positive rate among different duration groups and the association between the duration and the level of antibody were analyzed by chi-square test. Multiple pairwise rates comparison were analyzed by chi-square test combined with Bonferroni correction. The difference of average antibody titer among different duration groups was analyzed by one-way ANOVA and multiple pairs comparison was performed by LSD-t. Results Significant difference of positive rate was observed among different duration groups, P＝0.027，r＝0.112. Difference of positive rate between the 1～5 years (including 5 years) group and the 5～10 years (including 10 years) group was significant, P＝0.007. Further comparing the positive rate of patients within 5 years’ duration with those over 5 years’ duration indicated significant difference, P＝0.025，OR＝1.473，95％CI（1.049-2.068）. No significant difference on average antibody titre was observed among all groups. Conclusions Contrast to with the patients over 5 years’ duration, the positive rate of anti-tyrosinase IgG antibody in the patients within 5 years’ duration was higher, and which was more closely associated with duration of vitiligo patients. Therefore, duration of vitiligo patients was associated with the level of anti-tyrosinase IgG antibody in certain degree.

Key words: vitiligo, duration, monophenol monooxygenase, IgG antibody, association analysis