中华皮肤科杂志 ›› 2006, Vol. 39 ›› Issue (4): 181-183.

• 论著 •    下一篇

人毛囊内无色素黑素细胞的原子力显微镜观察

张汝芝1, 朱文元2, 鲍永军3, 夏明玉4, 马佳5   

  1. 1. 安徽省蚌埠医学院附属医院皮肤科 233004;
    2. 南京医科大学第一附属医院皮肤科;
    3. 南京大学物理系;
    4. 南京医科大学电镜室;
    5. 蚌埠医学院生物化学教研室
  • 收稿日期:2005-09-01 发布日期:2006-04-15
  • 基金资助:
    安徽省教育厅资助项目(2005kj048zd)

Observation of cultured amelanotic melanocytes from hair follicles by atomic force microscopy

ZHANG Ru-zhi1, ZHU Wen-yuan2, BAO Yong-jun3, X1A Ming-yu4, MA Jia5   

  1. Department of Dermatology, Affiliated Hospital of Bengbu Medical University, Bengbu 233004, Anhui, China
  • Received:2005-09-01 Published:2006-04-15

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摘要： 目的 用原子力显微镜观察人头发毛囊内无色素黑素细胞的表面结构。方法 分离酶Ⅱ消化带毛囊的头皮,游离单个毛囊,以胰酶/EDTA消化之,获取细胞悬液,以添加黑素细胞生长物质的254培养基重悬细胞,常规培养。传第3代后接种到内置云母片的6孔培养板中,细胞贴附到云母片上后,固定,原子力显微镜常温常压下,触摸式扫描。结果 毛囊无色素性黑素细胞多呈梭形,有2个突起,少数有3个树突。每个树突无明显的二级分枝,个别在树突侧缘有隆起。在一级树突的顶端可见丝状伪足。结论 毛囊内无色素性黑素细胞形态不成熟,不能转运黑素颗粒到角质形成细胞,但它有黑素小体运动的物质基础——丝状伪足。

关键词: 毛囊, 黑素细胞, 显微镜检查,原子力

Abstract: Objective To observe the surface structure of amelanotic melanocytes (AMMCs) from human hair follicles by atomic force microscopy (AFM). Methods Normal human scalp with hair follicles was incubated with dispase II. Individual hair follicles were isolated, then digested with pancre-atin/EDTA, and hair follicle cells were suspended and cultured in medium 254 supplemented with human melanocyte growth supplement. The cells of third passage were seeded in six-well plates, in which sheets of mica had been placed prior to the seeding. After adhered to the mica, the cells were fixed, then scanned and observed under normal atmospheric pressure and temperature by AFM in contact and tapping mode. Results Most cultured AMMCs from human hair follicles appeared fusiform with two dendrites, and rarely with three dendrites. The perikarya of AMMCs were round, oval or triangular. The dendrites had no clear secondary branches, while some had projections on the lateral sides. Filopodias were seen on the top of primary dendrites. Conclusion AMMCs from the human hair follicles are morphologically immature, might not take part in transferring melanosomes into keratinocytes, but they possess filopodias, the foundation for melanosome movement.

Key words: Hair follicle, Melanocytes, Microscopy, atomic force