中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (4): 278-280.

• 论著 • 上一篇    下一篇

银屑病患者角质形成细胞对朗格汉斯细胞分化发育的影响

唐玲1, 陶苏江1, 于益芝2, 王闻雅2, 刘书逊2, 顾军1, 郑茂荣1   

  1. 1. 第二军医大学附属长海医院皮肤科;
    2. 第二军医大学免疫学研究所
  • 收稿日期:2001-06-20 出版日期:2002-08-15 发布日期:2002-08-15
  • 基金资助:
    国家自然科学基金(39825123,39970689);上海市科技启明星计划资助(99QB14047)

The Effects of Psoriatic Lesional Keratinocytes on Development and Differentiation of Monocytes into Langerhans Cells

TANG Ling1, TAO Sujiang1, YU Yizhi2, WANG Wenya2, LIU Shuxun2, GU Jun1, ZHENG Maorong1   

  1. Department of Dermatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2001-06-20 Online:2002-08-15 Published:2002-08-15

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摘要： 目的 从朗格汉斯细胞(LC)的分化发育过程探讨银屑病角质形成细胞在此过程中的作用.方法 从健康者外周血分离单一核细胞,经银屑病患者角质形成细胞培养上清液与白介素4+粒细胞-巨噬细胞集落刺激因子(GM-CSF)+转化生长因子β1共同培养5d,使其分化发育为LC.通过流式细胞仪分析其表型、吞噬功能,通过混合淋巴细胞反应分析其抗原递呈功能.结果 银屑病患者角质形成细胞培养上清液与白介素4+GM-CSF+转化生长因子β1共同培养的LC其表型特征、吞噬抗原的能力以及刺激T淋巴细胞的活性等与健康对照组间差异无显着性.结论 银屑病患者角质形成细胞与正常人角质形成细胞对LC分化发育的影响差异无显着性,提示银屑病患者LC的异常可能不是发生于LC的分化发育过程中.

关键词: 银屑病, 角蛋白细胞, 朗格尔汉斯细胞, 细胞分化

Abstract: Objective To investigate the effects of psoria tic lesional keratinocytes on development and differentiation of monocytes into L angerhans cells(LCs)so as to analyze the mechanism of abnormality of LCs in patients with psoriasis.Methods Monocytes isolated from normal peripheral blood were cultured with granulocyte/macrophage colony-stimulating factor(GM-CSF),interleukin-4(IL-4),transforming growth factorβ1(TGF-β1)and supernatants from healthy or pso riatic lesional keratinocytes for 5days.Their phenotypes,phagocytic capacity were analyzed by FACS calibur.Their immunostimula tory capacity was detected by mixed lymphocytes reaction(MLR).Results The phenotypes,phagocytic capacity and immunostimulatory capacity we re almost uniform in PBMC-derived LCs cultured with GM-CSF,IL-4,TGF-β1 in combination with supernatants from keratinocytes between psoriatic lesions and normal controls.Conclusions In this experiment,no significant difference is observed in the effects of healthy and psoriatic lesional keratinocytes on development and differentiation of monocytes into LCs.The data sugge st that the abnormalities of LCs in patients with psoriasis might not take place in the stage of LC differentiation.

Key words: Psoriasis, Keratinocytes, Langerhans cells, Cell differentiation