中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (4): 276-277.

• 论著 • 上一篇    下一篇

寻常型银屑病患者外周血环氧合酶同工酶的研究

贾国泉, 陈志强, 刘训荃   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所
  • 收稿日期:2001-07-25 出版日期:2002-08-15 发布日期:2002-08-15
  • 通讯作者: 陈志强,中国医学科学院、中国协和医科大学皮肤病研究所,南京,210042

Expression of Cyclooxygenase mRNA in Peripheral Blood Mononuclear Cells in Patients with Progressive Psoriasis

JIA Guoquan, CHEN Zhiqiang, LIU Xunquan   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042, China
  • Received:2001-07-25 Online:2002-08-15 Published:2002-08-15

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摘要： 目的 探讨进行期斑块状银屑病患者外周血单一核细胞(PBMC)中环氧合酶同工酶的表达及其在银屑病发病机制中的作用.方法 应用逆转录-聚合酶链反应(RT-PCR)检测了进行期斑块状银屑病患者及健康对照者各30例PBMC中构成性环氧合酶(COX-1)和诱导性环氧合酶(COX-2)mRNA的表达.结果 进行期斑块状银屑病患者PBMC中COX-2mRNA的平均表达水平明显高于健康对照组(P<0.01),且其mRNA的表达水平与其相应的面积及严重度积分(PASI)呈正相关(P<0.01);而两组间COX-1平均表达水平的差异无显着性(P>0.05).结论 进行期斑块状银屑病患者PBMC中COX-2mRNA的表达增高,提示可能通过诱导前列腺素(PGs)的产生参与进行期斑块状银屑病的发病.

关键词: 银屑病, 前列腺素内过氧化物合酶

Abstract: Objective To investigate the expression levels of constitutive cyclooxygenase(COX-1)and inducible cyclooxygenase(COX-2)in progressive plaque psoriasis and their clinical significance.Methods Reverse transcription-polymerase ch ain reaction(RT-PCR)technique was applied to semi-quant itatively analyze COX-1 and COX-2mRNA expression in peripheral blood mononuclear cells(PBMC)from 30 psoriatic patients and 30 healthy controls,and the correlation between COX-1/-2 and psoriatic area and severity index(PASI)was evaluated.Results There was a significant difference o f COX-2 mRNA expression level betwee n psoriatic patients and normal controls(P<0.01),but no such difference was found reg arding COX-1 expression(P>0.05).Apositive correlation was noticed between COX-2 and PASI (r=0.68,P<0.01),but not between COX-1 and PASI.Conclusion The increased expression of COX-2 can stimulate over-production of prostaglandins and may be involved in the pathogenesis of progressive plaque psoriasis.

Key words: Psoriasis, Prostaglandin-endoperoxide synthase