中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (4): 281-283.

• 论著 • 上一篇    下一篇

副肿瘤性天疱疮患者免疫荧光与免疫印迹检测研究

李丽, 朱学骏, 卜定方, 秦俭, 陈喜雪   

  1. 北京大学第一医院皮肤性病科 100034
  • 收稿日期:2001-08-04 出版日期:2002-08-15 发布日期:2002-08-15

Immunofluorescent and Immunoblotting Features of Paraneoplastic Pemphigus

LI Li, ZHU Xuejun, BU Dingfang, QIN Jian, CHEN Xixue   

  1. Department of Dermatology, First Hospital, Peking University, Beijing 100034, China
  • Received:2001-08-04 Online:2002-08-15 Published:2002-08-15

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摘要： 目的 研究副肿瘤性天疱疮患者的免疫学特点.方法 采用免疫荧光和免疫印迹方法检测4例副肿瘤性天疱疮患者肿瘤切除前、后的血清.结果 以鼠膀胱为底物行间接免疫荧光示IgG和C3棘细胞间沉积,肿瘤切除后抗体滴度较术前下降,且与病情的好转成正比.以正常人皮肤及大鼠舌、食管及猴舌为底物行间接免疫荧光阳性;以大鼠支气管、心肌、肝脏、肾脏等组织为底物行间接免疫荧光阴性.免疫印迹示患者血清可识别人角质形成细胞提取物中的210000和190000抗原.结论 以鼠膀胱为底物的间接免疫荧光可作为副肿瘤性天疱疮的过筛试验,且可通过抗体滴度的改变监测病情的变化.副肿瘤性天疱疮更易累及粘膜部位上皮组织.免疫印迹结果可确诊4例患者为副肿瘤性天疱疮.

关键词: 天疱疮,副肿瘤性, 荧光抗体技术,间接, 免疫印迹法

Abstract: Objective To investigate the immunofluoresce nt and immunoblotting features of paraneoplastic pemphigus(PNP).Methods Sera were tested by indirect immunofluorecence(IIF)and immunoblotting(IB)in four patients with PNP.Results Binding of IgGand C3 to the intercell ular spaces of epidermis was found in IIF with rat bladder as substrate.The titers decreased after the excision of tumo rs.The autoantibodies also bound to substrates of the epithelia of human skin,rat tongue and esophagus in those p atients.The patients' sera recogni zed 210kD and 190kD antigens from human kera tinocyte extracts with IB analysis.Conclusions IIF with rat bladder as substrate can be used a screening test for PNP.PNP tends to involve epithelia of mucosa.The results of IB test m ay confirm the diagnosis of PNP of the 4 patients.

Key words: Pemphigus,paraneoplastic, Fluorescent antibody technique,indirect, Immunoblotting