中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (6): 383-385.

• 论著 • 上一篇    下一篇

银屑病患者表皮及真皮中Bcl-X、Bcl-2、Bax表达的免疫组化研究

茅晓红, 程浩, 孙国均, 张家敬, 周凯   

  1. 浙江医科大学附属第二医院皮肤科 杭州 310009
  • 收稿日期:1998-10-12 修回日期:1999-03-17 出版日期:1999-12-15 发布日期:1999-12-15
  • 基金资助:
    浙江省教委科研项目

Immunohistochemical study of Epidermal and Dermal Expression of Bcl-X, Bcl-2 and Bax in Psoriasis

MAO Xiaohong, CHENG Hao, SUN Cuojun   

  1. Department of Dermatology, Second Affiliated Hospital, Zhejiang Medical University, Hangzhou 310009
  • Received:1998-10-12 Revised:1999-03-17 Online:1999-12-15 Published:1999-12-15

PDF

294

赞

摘要： 目的 探讨银屑病患者细胞增殖与凋亡的调节.方法 用免疫组化SP法检测银屑病皮损及非皮损中Bcl-X、Bcl-2、Bax的表达情况.结果 银屑病皮损中,Bcl-X在表皮各层、真皮炎症细胞及血管内皮的表达均较正常明显增高;Bax在颗粒层与棘层的表达轻度增高.上述异常在非皮损中已经部分存在,且治疗后未完全恢复正常.而Bcl-2表达与正常相似,局限于基底层黑素细胞,角质形成细胞未见阳性.结论 银屑病中角质形成细胞过度增殖,炎症细胞浸润及血管增生可能与Bcl-X/Bax比率的上调有关.Bcl-2对银屑病的发病可能意义不大.

关键词: 银屑病, 细胞死亡, Bcl-X, Bcl-2, Bax

Abstract: Objective To investigate the regulation of cell proliferation and apoptosis in psoriasis.Methods The expressions of Bcl-X, Bcl-2 and Bax were studied with immunohistochemical technique(SP) in the lesional and non-lesional psoriatic skin.Results There were significant overexpressions of Bcl-X in all layers of epidermis, inflammatory cells and vascular endothelia in dermis; there were mild up-regulations of Bax expression in stratum granulosum and stratum spinosum in the lesional skin.Those abnormalities had already been observed in part of non-lesional skin and not returned to normal afer treatment.However, the expression of Bcl-2was as normal, restricted only to melanocytes in the basal layer; and no Bcl-2 expression was found in keratinocytes.Conclusion The up-regulated ratio of Bcl-X to Bax probably contributes to the overproliferation of keratinocytes, intensive infiltration of inflammatory cells and hyperplasia of new vessels.Bcl-2 may be less important for the initiation of psoriasis.

Key words: Psoriasis, Apoptosis, Bcl-X, Bcl-2, Bax