中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (6): 386-388.

• 论著 • 上一篇    下一篇

进行期银屑病患者皮损中胰岛素样生长因子Ⅰ受体蛋白与mRNA的表达

肖征1, 高进1, 王椿森2, 胡长发1   

  1. 1. 武汉市第一医院皮肤病研究室 武汉 430022;
    2. 同济医科大学附属协和医院皮肤科
  • 收稿日期:1998-11-12 修回日期:1999-07-26 出版日期:1999-12-15 发布日期:1999-12-15

Expression of Insulin-like Growth Factor Ⅰ Receptor Protein and mRNA in Active Psoriasis

XIAO Zheng1, CAO Jin1, WANG Chunsen2   

  1. The Investigative Laaboratory of Dermatology, First Hospital of Wuhan, 430022
  • Received:1998-11-12 Revised:1999-07-26 Online:1999-12-15 Published:1999-12-15

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摘要： 目的 探讨胰岛素样生长因子Ⅰ受体(IGFIR)和进行期银屑病的关系.方法 应用免疫组织化学技术,逆转录酶聚合酶链反应和原位杂交分别检测15例银屑病患者和10例正常人皮肤组织块中IGFIR蛋白与mRNA的表达.结果 IGFIR蛋白与mRNA表达在银屑病患者基底层和棘细胞层,IGFIR蛋白与mRNA相对积分光密度值在正常对照组与银屑病组间存在极显著性差异(P<0.01).结论 进行期银屑病患者皮损中表达增加的IGFIR蛋白和mRNA可能介导了角质形成细胞的增殖与分化不全.

关键词: 银屑病, 受体,胰岛素样生长因子Ⅰ, RNA,信使

Abstract: Objective To study the relationship between insulin-like growth factor Ⅰ receptor(IGFⅠ R)and active psoriasis.Methods The skin biopsies from active psoriatic lesions in fifteen cases with psoriasis and ten normal persons were investigated by immunohistochemical technique, RT-PCR and in situ hybridization.Results The expressions of IGF IR protein and mRNA were located on the basal cell layers and prickle cell layers.There was significant difference of mRNA expression between the normal and psoriatic groups(P<0.01).Condusion The increased expression of IGF IR protein and mRNA may mediate the hyperplasia and parakeratosis of keratinocytes in active psoriasis.

Key words: Psoriasis, Receptors,insulin-like-growth factorⅠ, RNA,messenger