中华皮肤科杂志 ›› 2018, Vol. 51 ›› Issue (6): 451-453.doi: 10.3760/cma.j.issn.0412-4030.2018.06.014

• 研究报道 • 上一篇    下一篇

泛发性脓疱性银屑病患者外周血单个核细胞中程序性细胞死亡受体1及其下游因子相关表达研究

朱腾1,晋红中2   

  1. 1. 中国医学科学院 北京协和医学院, 北京协和医院皮肤科
    2. 中国医学科学院北京协和医学院北京协和医院
  • 收稿日期:2017-04-18 修回日期:2017-11-29 发布日期:2018-05-30
  • 通讯作者: 晋红中 E-mail:jinhongzhong@126.com.cn
  • 基金资助:
    北京市自然科学基金;国家自然科学基金;首都卫生发展科研专项;中国医学科学院医学与健康科技创新工程项目

Expression of programmed cell death 1 receptor and its downstream cytokines in peripheral blood mononuclear cells of patients with generalized pustular psoriasis

Teng ZHU1,   

  • Received:2017-04-18 Revised:2017-11-29 Published:2018-05-30
  • Supported by:
    Beijing Municipal Natural Science Foundation;National Natural Science Foundation of China;Capital Funds for Health Improvement and Research;CAMS Initiative for Innovative Medicine

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摘要： 目的 检测泛发性脓疱性银屑病（GPP）患者外周血单个核细胞（PBMC）中程序性细胞死亡受体1（PDCD1）及其配体，以及下游NF?κB的表达。方法 采用Trizol法提取9例GPP患者及10例健康对照者PBMC中总RNA，应用逆转录PCR检测PDCD1、PD?L1、PD?L2及NF?κB mRNA表达水平。采用酶联免疫吸附试验检测23例GPP患者及24例健康对照者外周血血清白细胞介素（IL）?17、IL?22及IL?4含量。结果 GPP组PDCD1 mRNA相对表达量为3.13 ± 4.62，健康对照组为23.70 ± 15.33，两组比较，t = 3.40，P < 0.05。GPP组PD?L1、PD?L2相对表达量分别为（5.7 0 ± 3.07） × 10?4、（0.95 ± 0.71） × 10?4，健康对照组分别为（2.00 ± 1.98） × 10?4、（0.16 ± 0.20） × 10?4，两组差异均有统计学意义（t值分别为2.96、3.38，均P < 0.05）。两组间NF?κB mRNA相对表达量差异无统计学意义（P > 0.05）。患者组IL?4、IL?17、IL?22表达量均显著高于对照组（均P < 0.001）。结论 PDCD1及其配体在GPP患者PBMC中异常表达，可能在GPP的发生发展中扮演重要的角色。

关键词: 银屑病, 程序性细胞死亡受体1, 程序性细胞死亡1配体2蛋白, 抗原, CD274, NF-κB

Abstract: Zhu Teng, Jin Hongzhong Department of Dermatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China Corresponding author: Jin Hongzhong, Email: jinhongzhong@263.net 【Abstract】 Objective To determine the of programmed cell death 1 receptor （PDCD1）, its ligands and downstream nuclear factor kappa B （NF-κB） in peripheral blood mononuclear cells （PBMC） of patients with generalized pustular psoriasis （GPP）. Methods The total RNA was extracted from 9 GPP patients and 10 healthy controls separately by using Trizol, and reverse transcription PCR （RT-PCR） was performed to determine the mRNA of PDCD1, programmed cell death 1 ligand 1 （PD-L1）, PD-L2 and NF-κB. Enzyme-linked immunosorbent assay （ELISA） was conducted to the serum levels of interleukin-27 （IL-27）, IL-22 and IL-4 in the peripheral blood of 23 GPP patients and 24 healthy controls. Results The mRNA of PDCD1 was significantly lower in the GPP group （3.13 ± 4.62） than in the healthy control group （23.70 ± 15.33, t = 3.40, P < 0.05）. However, the mRNA of PD-L1 and PD-L2 was significantly higher in the GPP group than in the healthy control group （PD-L1: ［5.70 ± 3.07］ × 10-4 vs. ［2.00 ± 1.98］ × 10-4, t = 2.96, P < 0.05; PD-L2: ［0.95 ± 0.71］ × 10-4 vs. ［0.16 ± 0.20］ × 10-4, t = 3.38, P < 0.05）. There was no significant difference in the mRNA of NF-κB between the GPP group and the healthy control group （P > 0.05）. The GPP group also showed significantly higher levels of IL-27, IL-22 and IL-4 compared with the healthy control group （all P < 0.001）. Conclusion PDCD1 and its ligands were abnormally expressed in the PBMC of patients with GPP, and may play an important role in the occurrence and development of GPP.

Key words: Psoriasis, Programmed cell death 1 receptor, Programmed cell death 1 ligand 2 protein, Antigens, CD274, NF-kappa B

引用本文

朱腾 晋红中. 泛发性脓疱性银屑病患者外周血单个核细胞中程序性细胞死亡受体1及其下游因子相关表达研究[J]. 中华皮肤科杂志, 2018,51(6):451-453. doi:10.3760/cma.j.issn.0412-4030.2018.06.014

Teng ZHU. Expression of programmed cell death 1 receptor and its downstream cytokines in peripheral blood mononuclear cells of patients with generalized pustular psoriasis[J]. Chinese Journal of Dermatology, 2018, 51(6): 451-453.doi:10.3760/cma.j.issn.0412-4030.2018.06.014