中华皮肤科杂志 ›› 2018, Vol. 51 ›› Issue (6): 453-455.doi: 10.3760/cma.j.issn.0412-4030.2018.06.015

• 研究报道 • 上一篇    下一篇

12种抗菌药物对痤疮丙酸杆菌的体外抗菌活性研究

江丽,刘倩,张新翠,龚春燕   

  1. 中国医学科学院皮肤病医院
  • 收稿日期:2017-04-27 修回日期:2017-08-28 发布日期:2018-05-30
  • 通讯作者: 龚春燕 E-mail:13057687408@163.com
  • 基金资助:
    2015年中西医结合（CAIM）?澳美中国研究基金（痤疮研究）科研课题资助

Evaluation of in vitro antibacterial activity of 12 antibacterial agents against Propionibacterium acnes

Li JIANG1, 2, 1,Chun-Yan GONG   

  • Received:2017-04-27 Revised:2017-08-28 Published:2018-05-30
  • Contact: Chun-Yan GONG E-mail:13057687408@163.com
  • Supported by:
    CAIM-Bright Future of China Research Fund （Acne Research） in 2015

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摘要： 目的 评价12种抗菌药物对临床分离的痤疮丙酸杆菌（P.acnes）的体外抗菌活性。方法 本院2014年8月至2016年4月收集的100株P.acnes，采用肉汤稀释法测定其对四环素、多西环素、米诺环素、红霉素、罗红霉素、克拉霉素、阿奇霉素、甲氧苄啶、左氧氟沙星、氯霉素、克林霉素和夫西地酸的敏感率和最低抑菌浓度（MIC）。结果 米诺环素对P.acnes的MIC90与敏感率分别为8.0 mg/L、66%，多西环素为32 mg/L、36%，左氧氟沙星为16 mg/L、34%，氯霉素为128 mg/L、17%，克林霉素为 > 128 mg/L、7%；夫西地酸为16 mg/L、6%；四环素为 > 128 mg/L、4%，甲氧苄啶和阿奇霉素为 > 128 mg/L、3%；红霉素、罗红霉素和克拉霉素为> 128 mg/L、0。结论 临床分离的P.acnes对12种抗菌药物的敏感性由高到低为米诺环素、多西环素、左氧氟沙星、氯霉素、克林霉素、夫西地酸、四环素、甲氧苄啶、阿奇霉素，对红霉素、罗红霉素和克拉霉素耐药。

关键词: 痤疮丙酸杆菌, 抗菌药, 微生物敏感性试验

Abstract: Jiang Li, Liu Qian, Zhang Xincui, Gong Chunyan Department of Pharmacy, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China （Jiang L, Gong CY）; Department of Integrated Traditional Chinese and Western Medicine, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China（Liu Q, Zhang XC） Corresponding author: Gong Chunyan, Email: 13057687408@163.com 【Abstract】 Objective To evaluate the in vitro antibacterial activity of 12 antibacterial agents against clinically isolated Propionibacterium acnes （P.acnes）. Methods Totally, 100 strains of P.acnes were clinically isolated from Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College between August 2014 and April 2016. A broth dilution method was used to investigate the sensitivity rate of P.acnes isolates to 12 antibacterial agents including tetracycline, doxycycline, minocycline, erythromycin, roxithromycin, clarithromycin, azithromycin, trimethoprim, levofloxacin, chloramphenicol, clindamycin and fusidic acid, and determine the minimal inhibitory concentration （MIC） of the 12 antibacterial agents against P.acnes isolates. Results The MIC90 values of minocycline, doxycycline, levofloxacin, chloramphenicol, clindamycin, fusidic acid and tetracycline against P.acnes were 8, 32, 16, 128, > 128, 16 and > 128 mg/L, respectively, and the sensitivity rates of P.acnes to the 7 antibacterial agents were 66%, 36%, 34%, 17%, 7%, 6% and 4% respectively. Trimethoprim and azithromycin showed the MIC90 value of > 128 mg/L and sensitivity rate of 3%. Erythromycin, roxithromycin and clarithromycin showed the MIC90 value of > 128 mg/L and sensitivity rate of 0. Conclusion The clinically isolated P.acnes strains showed the highest sensitivity to minocycline, followed by doxycycline, levofloxacin, chloramphenicol, clindamycin, fusidic acid, tetracycline, trimethoprim and azithromycin, and were resistant to erythromycin, roxithromycin, and clarithromycin.

Key words: Propionibacterium acnes, Anti-bacterial agents, Microbial sensitivity tests

引用本文

江丽 刘倩 张新翠 龚春燕. 12种抗菌药物对痤疮丙酸杆菌的体外抗菌活性研究[J]. 中华皮肤科杂志, 2018,51(6):453-455. doi:10.3760/cma.j.issn.0412-4030.2018.06.015

Li JIANG Chun-Yan GONG. Evaluation of in vitro antibacterial activity of 12 antibacterial agents against Propionibacterium acnes[J]. Chinese Journal of Dermatology, 2018, 51(6): 453-455.doi:10.3760/cma.j.issn.0412-4030.2018.06.015