中华皮肤科杂志 ›› 2022, Vol. 55 ›› Issue (1): 55-57.doi: 10.35541/cjd.20200702

• 研究报道 • 上一篇    下一篇

小檗碱体外对酵母相马尔尼菲篮状菌的抗菌活性检测

罗宏1    潘开素2    张莲1    谈园1    黄敖1    曹存巍2   

  1. 1长沙市第一医院皮肤科，长沙  410005；2广西医科大学第一附属医院皮肤科，南宁  530021
  • 收稿日期:2020-07-10 修回日期:2021-07-26 发布日期:2021-12-31
  • 通讯作者: 曹存巍 E-mail:caocunwei@yeah.net
  • 基金资助:
    国家自然科学基金（81960567）；长沙市卫生计生委2018年科研计划课题项目

Evaluation of in vitro antifungal activity of berberine against Talaromyces marneffei in yeast phase

Luo Hong1, Pan Kaisu2, Zhang Lian1, Tan Yuan1, Huang Ao1, Cao Cunwei2   

  1. 1Department of Dermatology, The First Hospital of Changsha, Changsha 410005, China; 2Department of Dermatology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China
  • Received:2020-07-10 Revised:2021-07-26 Published:2021-12-31
  • Contact: Cao Cunwei E-mail:caocunwei@yeah.net
  • Supported by:
    National Natural Science Foundation of China （81960567）; Scientific Research Project of Changsha Municipal Health and Family Planning Commission in 2018

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摘要： 【摘要】 目的 体外检测小檗碱对酵母相马尔尼菲篮状菌（TM）的抗菌活性。方法 TM标准株（ATCC22019）1株，临床分离株10株，野生竹鼠分离株10株。TM以（1～5） × 103菌落形成单位/ml菌悬液接种于用微量稀释法制备的小檗碱、氟康唑、伊曲康唑、伏立康唑、两性霉素B、卡泊芬净药敏板，在37 ℃下培养48 h后观察结果，同时设阳性对照（仅加TM和培养基，不加药物）和阴性对照（仅加培养基，不加TM和药物）。参照M27?A3方案测定最低抑菌浓度（MIC）。结果 质控株（ATCC22019）MIC均在参考范围内，阳性对照孔TM生长良好。小檗碱MIC范围为32 ~ 64 mg/L；氟康唑对非耐药株MIC为2 ~ 4 mg/L，对2株临床耐药株为128 mg/L；伊曲康唑MIC为0.06 ~ 0.125 mg/L，伏立康唑为0.06 ~ 0.125 mg/L，两性霉素B为1 ~ 2 mg/L，卡泊芬净为16 ~ 32 mg/L。结论 小檗碱具有抑制酵母相TM活性的作用。

关键词: 小檗碱, 真菌, 微生物敏感性试验, 马尔尼菲篮状菌

Abstract: 【Abstract】 Objective To evaluate the in vitro antifungal activity of berberine against Talaromyces marneffei（TM） in yeast phase. Methods There were 21 TM strains, including l standard strain（ATCC22019）, 10 clinical isolates and 10 isolates from wild bamboo rats. TM strain suspensions at a concentration of （1 - 5） × 103 colony-forming units/ml were incubated in microdilution plates containing difierent concentrations of berberine, fluconazole, itraconazole, voriconazole, amphotericin B or caspofungin at 37 ℃ for 48 hours. Meanwhile, the wells containing only culture media and TM strains but without antifungal drugs served as positive control group, and those containing only culture media served as the negative control group. The minimum inhibitory concentrations （MICs） of antifungal drugs against yeasts were determined according to the Clinical and Laboratory Standards Institute （CLSI） broth microdilution susceptibility method （M27-A3 Document）. Results The MICs of the above antifungal drugs were all within the reference ranges for the quality control strain （ATCC22019）, and TM strains grew well in the positive control wells. The MIC ranges of berberine, itraconazole, voriconazole, amphotericin B and caspofungin against TM strains were 32 - 64 mg/L, 0.06 - 0.125 mg/L, 0.06 - 0.125 mg/L, 1 - 2 mg/L and 16 - 32 mg/L respectively; the MIC range of fluconazole was 2 - 4 mg/L for non-resistant strains, and 128 mg/L for fluconazole-resistant clinical strains. Conclusion Berberine exhibits antifungal activity against TM in yeast phase.

Key words: Berberine, Fungi, Microbial sensitivity tests, Talaromyces marneffei

引用本文

罗宏 潘开素 张莲 谈园 黄敖 曹存巍. 小檗碱体外对酵母相马尔尼菲篮状菌的抗菌活性检测[J]. 中华皮肤科杂志, 2022,55(1):55-57. doi:10.35541/cjd.20200702

Luo Hong, Pan Kaisu, Zhang Lian, Tan Yuan, Huang Ao, Cao Cunwei. Evaluation of in vitro antifungal activity of berberine against Talaromyces marneffei in yeast phase[J]. Chinese Journal of Dermatology, 2022, 55(1): 55-57.doi:10.35541/cjd.20200702