中华皮肤科杂志 ›› 2017, Vol. 50 ›› Issue (10): 715-718.doi: 10.3760/cma.j.issn.0412-4030.2017.10.005

• 论著 • 上一篇    下一篇

MiRNA-373在皮肤鳞状细胞癌的表达及对侵袭的影响

夏永华 侯慧芳 李敏 张彩凤 刘冬 胡华 张孟杰 程赛   

  1. 453100 河南卫辉，新乡医学院第一附属医院皮肤科（夏永华、李敏、刘冬、胡华、张孟杰、程赛），消化内科（张彩凤）；新乡医学院基础医学院病理生理教研室（侯慧芳）
  • 收稿日期:2016-12-23 修回日期:2017-05-25 发布日期:2017-09-29
  • 通讯作者: 夏永华 E-mail:751195651@qq.com
  • 基金资助:
    河南省教育厅科学技术研究重点项目（2011A320017）；新乡医学院科研培育基金（2013QN118）

Expression of microRNA-373 in cutaneous squamous cell carcinoma and its effect on cell invasion

Xia Yonghua, Hou Huifang, Li Min, Zhang Caifeng, Liu Dong, Hu Hua, Zhang Mengjie, Cheng Sai   

  1. Department of Dermatology, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan, China（Xia YH, Li M, Liu D, Hu H, Zhang MJ, Cheng S）; Department of Gastroenterology, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan, China（Zhang CF）; Department of Pathophysiology, School of Basic Medical Sciences, Xinxiang Medical University, Weihui 453100, Henan, China（Hou HF）
  • Received:2016-12-23 Revised:2017-05-25 Published:2017-09-29
  • Contact: xia E-mail:751195651@qq.com
  • Supported by:
    Key Program for Science and Technology Research of Henan Educational Committee （2011A320017）; Scientific Research Cultivation Fund of Xinxiang Medical University（2013QN118）

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摘要： 目的 分析microRNA-373（miR-373）在皮肤鳞状细胞癌（鳞癌）组织和细胞中的表达，探讨对皮肤鳞癌细胞侵袭的影响。方法 实时 PCR检测皮肤鳞癌组织和细胞中miR-373的表达，将miR-373模拟物、miR-373抑制剂以及阴性对照miRNA转染皮肤鳞癌A431细胞，采用细胞侵袭实验分析miR-373表达下调对细胞侵袭的影响，采用Western 印迹分析miR-373表达下调对MMP-2和MMP-9蛋白表达的影响。结果 皮肤鳞癌组织（2.465 ± 0.218）和SCL-1细胞及A431细胞（1.864 ± 0.178，2.919 ± 0.277）中miR-373的表达水平显著高于瘤旁组织和细胞（1.000 ± 0.000），差异有统计学意义（P < 0.05）。在转移性的皮肤鳞癌患者（3.323 ± 0.344）中，miR-373的表达显著高于非转移组（1.914 ± 0.161），差异有统计学意义（t = 4.158，P < 0.01）。与未处理组和阴性对照组相比，miR-373模拟物显著增加皮肤鳞癌A431细胞中miR-373的表达水平和细胞的侵袭能力，而miR-373的抑制剂显著下调皮肤鳞癌A431细胞中miR-373的表达水平和细胞的侵袭，差异有统计学意义（均P < 0.05）。结论 MiR-373表达下调显著抑制皮肤鳞癌A431细胞的侵袭，并能显著下调MMP-2和MMP-9蛋白的表达。

关键词: 肿瘤, 鳞状细胞；皮肤；微RNAs；肿瘤转移；microRNA-373

Abstract: Xia Yonghua, Hou Huifang, Li Min, Zhang Caifeng, Liu Dong, Hu Hua, Zhang Mengjie, Cheng Sai Department of Dermatology, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan, China （Xia YH, Li M, Liu D, Hu H, Zhang MJ, Cheng S）; Department of Gastroenterology, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan, China （Zhang CF）; Department of Pathophysiology, School of Basic Medical Sciences, Xinxiang Medical University, Weihui 453100, Henan, China （Hou HF） Corresponding author: Xia Yonghua, Email: 751195651@qq.com 【Abstract】 Objective To investigate the of microRNA-373 （miR-373） in cutaneous squamous cell carcinoma （CSCC） tissues and cells, and to explore its effects on cell invasion. Methods Real-time PCR was performed to determine the of miR-373 in CSCC tissues and paralesional normal skin tissues, as well as in CSCC cell lines （A431 and SCL-1） and HaCaT cells. A431 cells were divided into 4 groups: miR-373 mimic group, miR-373 inhibitor group and negative control group which were transfected with miR-373 mimic, miR-373 inhibitor and negative control miRNA respectively, and untreated group receiving no treatment. Cell invasion assay was performed to evaluate effects of miR-373 downregulation on cell invasion. Western blot analysis was conducted to assess effects of miR-373 downregulation on the protein of matrix metalloproteinase-2 （MMP-2） and MMP-9. Results Expression of miR-373 was significantly higher in the CSCC tissues（2.465 ± 0.218） than in the paralesional normal skin tissues （1.000 ± 0.000, P < 0.05）, and higher in SCL-1 cells （1.864 ± 0.178） and A431 cells（2.919 ± 0.277） than in HaCaT cells （1.000 ± 0.000, P < 0.05）. Most notably, miR-373 was also markedly higher in metastatic CSCC tissues than in non-metastatic CSCC tissues （3.323 ± 0.344 vs. 1.914 ± 0.161, t = 4.158, P = 0.000 4）. Compared with the untreated group and negative control group, the miR-373 mimic group showed significantly increased miR-373 and invasive ability, while the miR-373 inhibitor group showed markedly decreased miR-373 and invasive ability （all P < 0.05）. Conclusion MiR-373 downregulation can significantly suppress the invasion of A431 cells, and obviously decrease the protein of MMP-2 and MMP-9.

Key words: Neoplasms, squamous cell, Skin, MicroRNAs, Neoplasm metastasis, MicroRNA-373

引用本文

夏永华 侯慧芳 李敏 张彩凤 刘冬 胡华 张孟杰 程赛. MiRNA-373在皮肤鳞状细胞癌的表达及对侵袭的影响[J]. 中华皮肤科杂志, 2017,50(10):715-718. doi:10.3760/cma.j.issn.0412-4030.2017.10.005

Xia Yonghua, Hou Huifang, Li Min, Zhang Caifeng, Liu Dong, Hu Hua, Zhang Mengjie, Cheng Sai. Expression of microRNA-373 in cutaneous squamous cell carcinoma and its effect on cell invasion [J]. Chinese Journal of Dermatology, 2017, 50(10): 715-718.doi:10.3760/cma.j.issn.0412-4030.2017.10.005