Chinese Journal of Dermatology ›› 2011, Vol. 44 ›› Issue (7): 509-510.

• Research reports • Previous Articles     Next Articles

Effects of tacrolimus on the expression of stem cell factor (SCF) mRNA in human HaCaT keratinocytes and c-kit mRNA in murine B16 melanoma cells

  

  • Received:2010-08-16 Revised:2011-02-23 Online:2011-07-15 Published:2011-07-12

Abstract:

Objective To investigate the effects of tacrolimus on the expression of SCF mRNA in kera-tinocytes and c-kit mRNA in melanoma cells. Methods Tacrolimus was used to treat cultured HaCaT kera-tinocytes and B16 melanoma cells for 48 hours. Subsequently, real-time fluorescence quantitative PCR was performed to determine the mRNA expression of SCF in HaCaT cells and of c-kit in B16 cells. Results A significant change was observed in the expression of SCF mRNA in HaCaT cells and c-kit mRNA in B16 cells treated with tacrolimus compared with untreated HaCaT cells and B16 cells, respectively (both P < 0.05). Conclusion Tacrolimus can upregulate SCF mRNA expression in HaCaT cells and c-kit mRNA expression in B16 cells.

Key words: melanocyte