中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (3): 193-195.

• 研究报道 • 上一篇    下一篇

成纤维细胞生长因子10单克隆抗体对豚鼠银屑病样模型的作用研究

梅向林1,夏建新2,牟妍1,王敬医1,李雪1,朱文静3,李福秋4,金仙花1,于凯3   

  1. 1. 吉林大学第二医院
    2. 长春市吉林大学第二医院皮肤科
    3. 吉林大学第二临床医学院
    4. 长春吉林大学第二医院皮肤科
  • 收稿日期:2012-03-05 修回日期:2012-11-19 出版日期:2013-03-15 发布日期:2013-03-01
  • 通讯作者: 夏建新 E-mail:jianxinxia2003@tom.com
  • 基金资助:
    吉林省科技厅

Effect of a monoclonal antibody against fibroblast growth factor 10 in a guinea pig model of psoriasis-like skin inflammation

  • Received:2012-03-05 Revised:2012-11-19 Online:2013-03-15 Published:2013-03-01

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摘要： 目的 探讨成纤维细胞生长因子（FGF10）单克隆抗体局部外用对银屑病豚鼠模型的治疗作用。方法 应用5%盐酸普萘洛尔搽剂外涂豚鼠耳背部皮肤，诱导银屑病样动物模型。分别设置空白组、模型组、丁酸氢化可的松治疗组、FGF10抗体高（0.188 g/L）、中（0.094 g/L）、低（0.063 g/L）剂量治疗组。治疗2周后观察银屑病样模型的病理变化。采用SPSS 13.0统计软件进行统计学分析，Baker评分比较应用秩和检验，单一核细胞计数及表皮厚度比较应用方差分析（ANOVA），多个样本均数的两两比较应用最少显著差法（LSD）。 结果 各治疗组上述各项检测指标均低于模型组（均P < 0.05）。FGF10抗体高剂量组炎细胞计数与空白组差异无统计学意义（t = 0.77，P > 0.05），其余各治疗组炎细胞计数高于空白组及FGF10抗体高剂量组（均P < 0.05）。FGF10抗体各治疗组表皮厚度均高于丁酸氢化可的松治疗组，差异均有统计学意义（均P < 0.05）。FGF10抗体各治疗组组间表皮厚度比较，差异无统计学意义（均P > 0.05）。结论 FGF10单克隆抗体对银屑病样豚鼠模型中异常病理改变有显著调节作用，能影响角质形成细胞增殖分裂，还能显著抑制银屑病模型中炎症反应。

关键词: 银屑病, 成纤维细胞生长因子, 抗体，单克隆, 模型，动物

Abstract: MEI Xiang-lin, XIA Jian-xin, MU Yan, WANG Jing-yi, LI Xue, ZHU Wen-jing, LI Fu-qiu, JIN Xian-hua, YU Kai. Department of Dermatology, Second Hospital of Jilin University, Changchun 130041, China Corresponding author: XIA Jian-xin, Email: jianxinxia2003@tom.com 【Abstract】 Objective To evaluate the therapeutic effect of local application of an anti-fibroblast growth factor 10（FGF10） monoclonal antibody （MoAb） on a guinea pig model of psoriasis-like skin inflammation. Methods A model of psoriasis-like skin inflammation was established by applying 5% propranolol hydrochloride emulsion to the dorsal skin of ears of 45 guinea pigs, which were then classified into 5 groups: model group receiving no treatment, hydrocortisone butyrate group, high-, medium- and low-dose MoAb groups receiving topical treatment with hydrocortisone butyrate, anti-FGF10 MoAb of 0.188 g/L, 0.094 g/L and 0.063 g/L, respectively, twice daily. Nine guinea pigs receiving no propranolol challenge nor treatment served as the blank control group. The response of guinea pigs to propranolol hydrochloride emulsion and therapeutic agents, such as scratch behavior, hair status and skin status, was observed and recorded. After two weeks of treatment, all the guinea pigs were sacrificed, and skin samples were resected from the ears followed by the evaluation of Baker score, measurement of epidermal thickness, and enumeration of mononuclear cells. SPSS 13.0 software was used for data processing. Baker score was compared by rank sum test, mononuclear cell count and epidermal thickness by analysis of variance. Pairwise comparisons were carried out by the least significant difference （LSD） procedure. Results The number of mononuclear cells was similar between the high-dose MoAb group and blank control group （t = 0.77, P > 0.05）, but higher in the other treatment groups than in the high-dose MoAb group and blank control group （all P < 0.05）. Increased epidermal thickness was observed in the three MoAb groups compared with the hydrocortisone butyrate group （all P < 0.05）, while there was no statistical difference between the three MoAb groups （all P > 0.05）. Conclusions The anti-FGF10 MoAb obviously attenuates the pathological changes, affects the proliferation of keratinocytes, and markedly suppresses the inflammation, in the psoriasis-like skin model in guinea pigs.

中图分类号: 

  • R751