中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (11): 784-787.

• 论著 • 上一篇    下一篇

治疗剂量窄谱中波紫外线对B10BR黑素细胞增殖、凋亡及黑皮素受体-1表达的影响

宋秀祖1,相文忠1,卢良君1,许爱娥2   

  1. 1. 杭州市第三人民医院皮肤科
    2. 安徽医科大学附属杭州市第三人民医院皮肤科
  • 收稿日期:2010-03-16 修回日期:2010-04-12 出版日期:2010-11-15 发布日期:2010-11-10
  • 通讯作者: 宋秀祖 E-mail:songxiuzu@sina.com.cn; songxiuzu@yahoo.com
  • 基金资助:

    皮肤屏障保护剂的前期开发研究;AQP3在皮肤角质形成细胞通透甘油中的作用机制研究

Effects of narrow band ultraviolet B at therapeutic doses on the proliferation of, apoptosis in and melanocortin-1 receptor （MC-1R） expression by melanocytes

Song XiuZuXiang WenZhong 2   

  • Received:2010-03-16 Revised:2010-04-12 Online:2010-11-15 Published:2010-11-10
  • Contact: Song XiuZu E-mail:songxiuzu@sina.com.cn; songxiuzu@yahoo.com

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摘要：

目的 通过观察311 nm窄谱中波紫外线（NB-UVB）对黑素细胞增殖、凋亡及黑素合成的影响，探讨NB-UVB在白癜风复色中的可能机制。方法 永生化B10BR黑素细胞，采用400、800、1200 mJ/cm2的311 nm NB-UVB照射，MTT法及流式细胞仪测定细胞增殖及凋亡变化，NaOH法测定黑素含量变化，RT-PCR方法测定B细胞淋巴瘤-2（BCL-2）表达变化，免疫细胞化学方法及Western印迹测定黑皮素受体-1（MC-1R）表达。结果 用不同剂量NB-UVB照射B10BR后，黑素细胞的增殖和凋亡没有显著变化。但随着NB-UVB照射剂量增加，黑素合成呈剂量依赖性增加，分别为正常对照组的1.42倍、1.78倍、2.05倍。BCL-2的表达显著增加，分别为正常对照组的1.75倍、2.32倍、3.28倍。黑素细胞MC-1R蛋白表达也显著增加，分别为对照组的1.68倍、2.35倍、3.01倍。结论 治疗剂量的311 nm NB-UVB不会导致黑素细胞凋亡，并且可以通过上调BCL-2及MC-1R的表达，增强黑素细胞的抗氧化应激能力，促进黑素合成。

关键词: 黑素细胞, 窄谱中波紫外线, 凋亡

Abstract:

Objective To investigate the mechanism of repigmentation in vitiligo induced by narrow band ultraviolet B（NB-UVB） via observing the effects of 311 nm NB-UVB on the proliferation of, apoptosis and melanogenesis in melanocytes. Methods Immortalized B10BR melanocytes were irradiated with NB-UVB at varying doses （400, 800 and 1200 mJ/cm2）. Subsequently, the proliferation of and apoptosis in melanocytes were detected by MTT assay and flow cytometry, respectively, and the content of melanin was determined by NaOH assay. The expression of BCL-2 mRNA was examined by RT-PCR, and MC-1R expression of melanocytes by immunocytochemistry and Western blotting. Results The proliferation of and apoptosis in melanocytes experienced no obvious change after irradiation with NB-UVB at the three tested doses. In B10BR melanocytes irradiated with NB-UVB at 400, 800 and 1200 mJ/cm2, the melanin content was 1.42, 1.78, 2.05 times, the mRNA expression of BCL-2 was 1.75, 2.32, 3.28 times, and the protein expression of MC-1R was 1.68, 2.35 and 3.01 times, that in unirradiated melanocytes, respectively. Conclusion NB-UVB irradiation at therapeutic doses could promote the melanogenesis in, enhance anti-oxidative stress activities of melanocytes, by upregulating the expressions of BCL-2 and MC-1R, with no marked effects on the apoptosis in melanocytes.

Key words: apoptosis, melanocyte, narrow band ultraviolet B