中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (9): 612-614.

• 论著 • 上一篇    下一篇

梅毒患者外周血树突细胞亚型及其表达不同细胞因子的检测

郭义龙 许敏鸿 蔡小丹 杨启平   

  1. 汕头市皮肤医院 汕头市皮肤性病防治院
  • 收稿日期:2008-03-03 修回日期:2008-05-21 发布日期:2008-09-15
  • 通讯作者: 郭义龙 E-mail:stgyl@126.com

Detection of peripheral dendritic cell subsets and T cell subpopulations expressing different intracellular cytokines in patients with syphilis

GUO Yi-Long   

  • Received:2008-03-03 Revised:2008-05-21 Published:2008-09-15
  • Contact: GUO Yi-Long E-mail:stgyl@126.com

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摘要： 目的 探讨梅毒患者外周血树突细胞（DC）各亚型及表达不同细胞因子T细胞亚群的频数在梅毒发病机制中的意义。方法 流式细胞仪测定20例梅毒患者治疗前、治疗后和15例正常人外周血DC各亚型及表达不同细胞因子（包括IFN-γ、IL-2、IL-4、IL-10、IL-12）CD4+、CD8+ T细胞亚群的频数。结果 20例梅毒患者外周血髓系DC亚型（CD11c＋）的表达，治疗前、后比较有显著升高（t = 3.339，P ＜ 0.01），治疗前与对照组比较显著升高（P ＜ 0.05）。梅毒患者外周淋巴系DC亚型（CD123＋）的表达，治疗前、治疗后、对照组比较其差异均无统计学意义（P ＞ 0.05）。梅毒患者外周血T细胞亚群胞质内细胞因子IFN-γ的表达，治疗前、后比较有显著升高（t =14.890，P ＜ 0.01），治疗前与对照组比较显著升高（P ＜ 0.01）；梅毒患者外周血T细胞亚群胞质内细胞因子IL-2、IL-4、IL-10、IL-12的表达，IL-2和IL-12治疗前、后比较有显著升高（t = 10.899，4.292，P均 ＜ 0.01），治疗前与对照组比较显著升高（P ＜ 0.05），IL-4和IL-10治疗前与对照组比较显著降低（P ＜ 0.01）。梅毒患者外周血CD11c＋、CD123＋的表达与IFN-γ、IL-12的百分比不存在相关关系（P均 ＞ 0.05）。结论 IFN-γ、IL-2和IL-12可能在梅毒清除过程中发挥免疫调节作用，而IL-4、IL-10起免疫抑制作用。

关键词: 梅毒, 树突状细胞, 亚型, T淋巴细胞, 细胞因子类

Abstract: Objective To investigate the role of dendritic cell （DC） subsets and T cell subpopulations expressing different intracellular cytokines in the pathogenesis of syphilis. Methods Flow cytometry was utilized to measure the frequencies of peripheral DC subsets and IFN-γ-, IL-2-, IL-4-, IL-10-expressing peripheral CD4+ and CD8+ T cell subpopulations in 20 patients with syphilis before and after therapy, and in 15 healthy human controls. Results The frequency of CD11c+ DC was significantly lower in pretreated patients than that in treated patients （P < 0.01）, but higher than that in the normal controls （P < 0.05）, and the same was true for the frequency of IFN-γ-expressing T cells, IL-2-expressing T cells, and IL-12-expressing T cells. By contrast, a decrease was noticed in the frequency of IL-4-expressing T cells and IL-10-expressing T cells in pretreated patients compared with that in the normal controls. Furthermore, in patients with syphilis, the frequency of CD11c+ and CD123+ DC was unrelated to the percentage of IFN-γ-expressing and IL-12- expressing T cells （both P > 0.05）. Conclusions IFN-γ, IL-2 and IL-12 may exert an immunoregulatory effect in the eliminating of Treponema pallidum, whereas IL-4 and IL-10 may perform an immunoinhibitory effect.

Key words: syphilis, dendritic cell, subset, T-lymphocytes, Cytokines