中华皮肤科杂志 ›› 2020, Vol. 53 ›› Issue (7): 546-550.doi: 10.35541/cjd.20191007

• 论著 • 上一篇    下一篇

基于TP0136蛋白异质性建立一种新的苍白密螺旋体分子分型方法

魏然1,柯吴坚2,3,陈文韬4,谭玲俏5,刘雅慧2,吕萍6,黄涛3,张君3,张晓辉3,王柳苑3,车雅敏1   

  1. 1. 天津医科大学总医院
    2. 广东省皮肤病医院
    3. 南方医科大学，广东省皮肤病医院
    4. 南方医科大学皮肤病医院
    5. 阳江市公共卫生医院
    6. 广州市广东省皮肤性病防治中心
  • 收稿日期:2019-10-21 修回日期:2020-04-09 发布日期:2020-07-06
  • 通讯作者: 车雅敏 E-mail:lily_alan@sohu.com
  • 基金资助:
    广东省医学科研基金;国家自然科学基金;深圳龙华区引进高层次医学团队项目;南方医科大学皮肤病医院基础研究苗圃项目;广州市健康医疗协同创新重大专项

Establishment of a new molecular typing method for Treponema pallidum based on TP0136 protein sequence heterogeneity

  • Received:2019-10-21 Revised:2020-04-09 Published:2020-07-06
  • Contact: CHE Ya-Min E-mail:lily_alan@sohu.com
  • Supported by:
    Medical Scientific Research Foundation of Guangdong Province of China;National Natural Science Foundation of China;Shenzhen Longhua District High-Level Medical Team Project;Basic Research Nursery Project of Dermatology Hospital of Southern Medical University;Guangzhou Healthcare Collaborative Innovation Major Project

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摘要： 【摘要】 目的 基于苍白密螺旋体属（TP）TP0136蛋白的异质性，建立一种新的TP分子分型方法。方法 从GenBank中查询9株梅毒苍白密螺旋体（TPA）、3株雅司苍白密螺旋体（TPE）、1株未分类的类人猿密螺旋体（FB）和1株地方密螺旋体（TEN）的TP0136开放阅读框（ORF）氨基酸序列并进行多重序列比对，得出TP0136蛋白分子分型结果。利用建立的TP0136蛋白分子分型方法，对2015年 1月至2018年12月南方医科大学皮肤病医院收集的23株TPA临床分离株进行分类，并将分型结果与传统的Arp/ Tpr/ TP0548三基因分型方法进行比较。结果 TP0136蛋白在不同种TP株中具有高度异质性，根据TP0136氨基酸序列，TPE、FB和TEN分为Ⅰ~ Ⅳ 4个亚型，TPA分为Ⅴ~ Ⅹ 6个亚型，TPA临床株分为Ⅶ、Ⅸ、Ⅹ、Ⅺ 4个亚型。通过TPA传统的三基因分型方法可将23株TPA临床株分为13D/d、14D/f、14D/g、15D/f、16A/e 5型。将TP0136分型法与传统分型法相结合，可将上述临床株进一步细分为10型，其中14D/f型应用TP0136分型法可进一步分为3个亚型。结论 TP0136分子分型方法可用于TP种的区分，有助于传统TPA分子分型的进一步完善。

关键词: 密螺旋体属, 梅毒, 基因分型技术, 序列分析, 蛋白质, TP0136

Abstract: 【Abstract】 Objective To establish a new molecular typing method for Treponema pallidum （TP） based on TP0136 protein sequence heterogeneity. Methods The amino acid sequences of TP0136 open reading frame （ORF） of 9 strains of Treponema pallidum ssp. Pallidum （TPA）, 3 strains of Treponema pallidum ssp. Pertenue （TPE）, 1 unclassified simian strain of Treponema Fribourg-Blanc （FB） and 1 strain of Treponema pallidum ssp. Endemicum （TEN） were searched from Genbank, and multiple sequence comparisons were performed to obtain the molecular typing results of TP0136 protein. The TP0136 protein-based molecular typing method was used to classify 23 TPA clinical isolates, which were collected from Dermatology Hospital of Southern Medical University from January 2015 to December 2018, and the typing results were compared with those by the traditional typing method based on the tp0548/Arp/Tpr genes. Results TP0136 protein was highly heterogeneous in different TP strains. According to the amino acid sequence of TP0136, TPE, FB and TEN strains were divided into 4 subtypes of Ⅰ- Ⅳ, TPA strains were divided into 6 subtypes of Ⅴ-Ⅹ, and TPA clinical strains were classified into 4 subtypes of Ⅶ, Ⅸ, Ⅹ, Ⅺ. Through the traditional typing method described above, 23 TPA clinical strains could be divided into 5 types （13D/d, 14D/f, 14D/g, 15D/f, 16A/e）. By using the TP0136 protein-based typing method combined with traditional typing method, the above clinical strains could be further subdivided into 10 types, and the 14D/f type could be further divided into 3 subtypes by using the TP0136 protein-based typing method. Conclusion The TP0136 protein-based molecular typing method can be used to distinguish TP species, which is helpful for further improvement of traditional TPA molecular typing.

Key words: Treponema, Syphilis, Genotyping techniques, Sequence analysis, protein, TP0136

引用本文

魏然 柯吴坚 陈文韬 谭玲俏 刘雅慧 吕萍 黄涛 张君 张晓辉 王柳苑 车雅敏. 基于TP0136蛋白异质性建立一种新的苍白密螺旋体分子分型方法[J]. 中华皮肤科杂志, 2020,53(7):546-550. doi:10.35541/cjd.20191007