中华皮肤科杂志 ›› 2022, Vol. 55 ›› Issue (9): 806-809.doi: 10.35541/cjd.20210780

• 研究报道 • 上一篇    下一篇

微RNA单核苷酸多态性与慢性自发性荨麻疹发病风险的关联分析

鲁楠    谭兴友    刘香    牛李莉    姚树兰   

  1. 济宁医学院附属医院皮肤科，济宁  272029
  • 收稿日期:2021-10-26 修回日期:2022-01-12 发布日期:2022-09-02
  • 通讯作者: 鲁楠 E-mail:lnnancy@163.com
  • 基金资助:
    济宁医学院教师科研扶持基金项目（JYFC2018FKJ052）

Association of single nucleotide polymorphisms in microRNAs with the risk of chronic spontaneous urticaria

Lu Nan, Tan Xingyou, Liu Xiang, Niu Lili, Yao Shulan   

  1. Department of Dermatology, Affiliated Hospital of Jining Medical University, Jining 272029, Shandong, China
  • Received:2021-10-26 Revised:2022-01-12 Published:2022-09-02
  • Contact: Lu Nan E-mail:lnnancy@163.com
  • Supported by:
    Supporting Fund for Teachers′ Research of Jining Medical University（JYFC2018FKJ052）

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摘要： 【摘要】 目的 探讨微RNA（miRNA）单核苷酸多态性与慢性自发性荨麻疹（CSU）的风险关联。方法 采用病例对照研究，收集2019年1 - 6月在济宁医学院附属医院皮肤科诊治的98例CSU患者及同期148例健康对照，均为山东籍汉族，采集静脉血后提取基因组DNA，针对miRNA多态性位点rs2431697（miR-146a）、rs57095329（miRNA-146a）、rs3746444（miRNA-499）、rs11614913（miRNA-196a2）和rs895819（miRNA-27a）行多重PCR扩增反应和单碱基延伸反应，进行单核苷酸多态性（SNP）位点分型。采用χ2检验分析组间等位基因、基因型及遗传模型分布差异，非条件Logistic回归分析基因SNP与疾病发生风险的关系。结果 所有样本SNP均成功分型。miRNA-196a2 SNP位点rs11614913等位基因为T/C，病例组等位基因T频数110（56.1%），对照组131（44.3%），两组T/C频率分布差异有统计学意义（χ2 = 6.64，P = 0.010），该位点等位基因T可能是CSU发生的危险因素（OR = 1.61，95％ CI：1.12 ~ 2.32）。病例组rs11614913基因型CC、CT、TT频数分别为16（16.3%）、54（55.1%）、28（28.6%），对照组为48（32.4%）、69（46.6%）、31（20.9%），两组基因型分布差异有统计学意义（χ2 = 8.16，P = 0.017）；两组显性遗传模型（TT + CT与CC）分布差异亦有统计学意义（χ2 = 7.95，P = 0.005），显性遗传模型可能增加了CSU发病风险（OR = 2.46，95％ CI：1.30 ~ 4.65）。结论 我国山东汉族人群miRNA-196a2 SNP与CSU风险可能存在关联，rs11614913可能会增加CSU发病风险。

关键词: 荨麻疹, 微RNAs, 多态性, 单核苷酸, 基因分型技术, rs11614913

Abstract: 【Abstract】 Objective To investigate the association between single nucleotide polymorphisms （SNPs） in microRNAs （miRNAs） and the risk of chronic spontaneous urticaria（CSU）. Methods A case-control study was conducted. A total of 98 patients with CSU （CSU group） were collected from Department of Dermatology, Affiliated Hospital of Jining Medical University from January to June in 2019, and 148 health checkup examinees （control group） were collected at the same time, all of whom were of Han population from Shandong province. Genomic DNA was extracted from venous blood samples, and polymorphic sites rs2431697 （miRNA-146a）, rs57095329 （miRNA-146a）, rs3746444 （miRNA-499）, rs11614913 （miRNA-196a2） and rs895819 （miRNA-27a） were analyzed for SNP genotyping by multiplex PCR amplification and single-base extension. Chi-square test was used to analyze differences in the distribution of alleles, genotypes and genetic models between the two groups, and unconditional Logistic regression to analyze the relationship between gene SNPs and the risk of CSU. Results All samples were successfully genotyped by analysis of the 5 polymorphic sites. The alleles of the miRNA-196a2 SNP rs11614913 were T/C, and the absolute frequency of T allele was 110 （56.1%） in the CSU group and 131（44.3%） in the control group; there was a significant difference in the T/C allele frequency distribution between the two groups （χ2 = 6.64, P = 0.010）, and the T allele might be a risk factor for CSU （OR = 1.61, 95％ CI: 1.12 - 2.32）. In addition, the absolute frequencies of CC, CT and TT genotypes of rs11614913 were 16 （16.3%）, 54 （55.1%）, 28 （28.6%） in the CSU group and 48 （32.4%）, 69 （46.6%）, 31 （20.9%） in the control group respectively, and there was a significant difference in the genotype distribution between the two groups （χ2 = 8.16，P = 0.017）; the distribution of the dominant genetic model （TT + CT vs. CC） also significantly differed between the two groups （χ2 =7.95, P = 0.005）, which might increase the risk of CSU （OR = 2.46, 95％ CI: 1.30 - 4.65）. Conclusion The miRNA-196a2 SNPs may be associated with the risk of CSU in the Han population in Shandong, China, and the rs11614913 polymorphism may increase the risk of CSU.

Key words: Urticaria, MicroRNAs, Polymorphism, single nucleotide, Genotyping techniques, rs11614913