中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (6): 384-386.

• 论著 • 上一篇    下一篇

雌激素对SLE患者T细胞 CD154 mRNA稳定性的影响

李晓岚 Virginia Rider Bruce Kimler Nabih I Abdou   

  1. 昆明医学院第二附属医院皮肤科 Pittsburg State University Pittsburg State University Pittsburg State University
  • 收稿日期:2007-09-11 修回日期:2007-12-11 发布日期:2008-06-15
  • 通讯作者: 李晓岚 E-mail:xlnli@yahoo.com,lixiaolan96@hotmail.com

Effects of estrogen on CD154 mRNA stability in T cells of patients with systemic lupus erythematosus

Virginia Rider   

  • Received:2007-09-11 Revised:2007-12-11 Published:2008-06-15

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摘要： 目的 探讨SLE患者T细胞CD154表达的雌激素依赖性增加是否与其mRNA的稳定性变化相关。方法 用不含或含有17-β 雌二醇（10-7 mol/L）的无血清培养液培养SLE女性患者（10例）及正常人对照（7例）的T细胞共18 h。取一部分T细胞放至用抗CD3包被的培养皿中进一步培养激活，并在平行培养中加入放线菌D以阻止新的mRNA合成。CD154 mRNA的稳定性用RT-PCR测定，并与不经刺激的T细胞（静止细胞）作对照。结果 静止的SLE T细胞在含雌二醇的培养液中CD154/G3PDH为36.9 ~ 100， = 75.5；不含雌二醇的CD154/G3PDH为14.5 ~ 100， = 73.6，两组比较，P = 0.88；正常T细胞分别为34.2 ~ 100， = 76.3和47 ~ 100， = 74.3，两组比较，P = 0.65。在对雌激素应答过程中，静止的SLE T细胞和正常T细胞mRNA的稳定性差异均无统计学意义。同样，激活的SLE T细胞在含雌二醇的培养液中CD154/G3PDH为34 ~ 100， = 86.8；不含雌二醇的CD154/G3PDH为28.5 ~ 100， = 54.5，两组比较，P = 0.15；正常T细胞分别为73 ~ 100， = 87.7和40 ~ 98， = 68.1，两组比较，P = 0.077。在雌激素的作用下，激活的SLE T细胞和正常T细胞CD154 mRNA的稳定性差异也无统计学意义。结论 SLE T细胞中CD154的雌激素依赖性表达增加并不是由mRNA稳定性变化引起

关键词: CD154, SLE, 雌激素, T细胞, mRNA稳定性

Abstract: Objective To investigate if the estrogen-dependent increase in CD154 expression in T cells of patients with systemic lupus erythematosus （SLE） is due to alterations in its mRNA stability. Methods T cells isolated from 10 female patients with SLE and 7 normal controls were cultured for 18 hours in serum-free medium with or without 10-7 mol/L of 17β-estradiol . Some of the T cells were then harvested and subjected to further culture in anti-CD3-coated plates for activation. Actinomycin D, 25 μg/mL, was added to the parallel cultures to inhibit further synthesis of mRNA. Unstimulated resting cells served as control. The expression of CD154 mRNA was assessed by reverse-transcription PCR. Results The average levels of CD154 mRNA relative to GAPDH mRNA were 75.5 and 73.6 in resting SLE T cells cultured with and without estradiol, respectively （P = 0.88）, 76.3 and 74.3 in resting normal T cells respectively （ P = 0.65）. No difference was observed in the stability of mRNA between SLE and normal control resting T cells in response to estradiol. After activation with anti-CD3 antibody, the average levels of CD154 mRNA relative to GAPDH mRNA were 86.8 and 54.5 in estradiol-stimulated and unstimulated SLE T cells respectively （P = 0.15）, 87.7 and 68.1 in normal T cells respectively （P = 0.077）. There was no statistical difference in the stability of mRNA between SLE and normal activated T cells stimulated by estradiol. Conclusion These findings suggest that the estrogen-dependent increase in CD154 of SLE T cells is not due to the alteration in stability of mRNA.

Key words: CD154, SLE, estrogen, T cell, mRNA stability