中华皮肤科杂志 ›› 2021, Vol. 54 ›› Issue (4): 364-367.doi: 10.35541/cjd.20190964

• 综述 • 上一篇    

过敏原特异性T细胞检测方法的研究进展

徐蓓蕾    姚煦   

  1. 中国医学科学院、北京协和医学院皮肤病研究所过敏与风湿免疫科,南京  210042 
  • 收稿日期:2019-10-08 修回日期:2020-04-25 发布日期:2021-03-31
  • 通讯作者: 姚煦 E-mail:dryao_xu@126.com
  • 基金资助:
    国家自然科学基金(81371735、81573045);江苏省自然科学基金(BK20150166);中国医学科学院医学与健康科技创新工程项目(2016-I2M-1-005)

Detection methods for allergen-specific T cells

Xu Beilei, Yao Xu   

  1. Department of Allergy and Rheumatology, Hospital of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China
  • Received:2019-10-08 Revised:2020-04-25 Published:2021-03-31
  • Contact: Yao Xu E-mail:dryao_xu@126.com
  • Supported by:
    National Natural Science Foundation of China(81371735, 81573045); Natural Science Foundation of Jiangsu Province (BK20150166); CAMS Innovation Fund for Medical Sciences (2016-I2M-1-005)

摘要: 【摘要】 追踪T细胞数量和功能的变化在临床诊断和评估过敏原免疫治疗疗效等方面具有重要的参考价值。本文综述了羧基荧光素琥珀碱亚胺酯连续稀释法、酶联免疫斑点检测法、胞内细胞因子染色法和微阵列免疫传感器等过敏原特异性T细胞检测方法和临床应用的研究进展,为选择和发展合适的检测方法并应用于临床提供参考。

关键词: T细胞抗原受体特异性, 主要组织相容性复合物, 酶联免疫斑点测定, 过敏性疾病, 过敏原特异性T细胞, 羧基荧光素琥珀碱亚胺酯连续稀释法, 胞内细胞因子染色法, 微阵列免疫传感器

Abstract: 【Abstract】 Tracking changes in the number and function of T cells is of great value to clinical diagnosis and evaluation of the efficacy of allergen immunotherapy. This review summarizes research progress in detection methods for allergen-specific T cells and their application, such as carboxyfluorescein succinimidyl ester dilution assay, enzyme-linked immunospot assay, intracellular cytokine staining assay and microarray immunosensors, providing references for selecting and developing appropriate detection methods in clinical practice.

Key words: T-cell antigen receptor specificity, Major histocompatibility complex, Enzyme-linked immunospot assay, Allergic diseases, Allergen-specific T cells, Carboxyfluorescein succinimidyl ester dilution assay, Intracellular cytokine staining assay, Microarray immunosensors