中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (7): 489-491.

• 论著 • 上一篇    下一篇

小干扰RNA沉默KIAA0101的表达对皮肤鳞状细胞癌SCL-1细胞增殖和迁移的影响

李敏1,夏永华2,刘冬2,付丹丹3,李占国4,田中伟5   

  1. 1. 新乡医学院第一附属医院
    2. 新乡医学院第一附属医院皮肤性病科
    3. 新乡医学院第一附属医院皮肤科
    4.
    5. 河南省新乡医学院第一附属医院皮肤科
  • 收稿日期:2012-09-10 修回日期:2012-10-29 出版日期:2013-07-15 发布日期:2013-07-01
  • 通讯作者: 田中伟 E-mail:zhonwt@163.com
  • 基金资助:
    河南省教育厅自然科学研究计划项目;河南省卫生科技创新型人才工程专项经费;新乡医学院重点研究领域招标课题资助

Effect of small interfering RNA?鄄induced silencing of KIAA0101 gene expression on the proliferation and invasion of a cutaneous squamous cell carcinoma cell line SCL-1

  • Received:2012-09-10 Revised:2012-10-29 Online:2013-07-15 Published:2013-07-01

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摘要： 目的 探讨KIAA0101蛋白表达下调对皮肤鳞状细胞癌（SCC）SCL-1细胞增殖和细胞侵袭能力影响的分子机制。方法 将KIAA0101 siRNA和对照siRNA分别转染SCL-1细胞，将SCL-1细胞分为3组：未转染组、对照siRNA组和KIAA0101 siRNA组。Western印迹检测3组细胞中KIAA0101蛋白的表达，CCK-8试剂检测细胞增殖，用Boyden小室检测细胞侵袭能力， Western印迹检测细胞增殖和细胞侵袭相关蛋白的表达。结果 KIAA0101 siRNA组中KIAA0101蛋白的相对表达量为0.062 ± 0.095，显著低于未转染组（0.359 ± 0.044）和对照siRNA组（0.379 ± 0.025），P < 0.05，SCL-1细胞的增殖和侵袭能力亦降低（P < 0.05）。此外，与未转染组和siRNA对照组相比，KIAA0101 siRNA组中p21蛋白表达显著上升，而基质金属蛋白酶2表达显著下调（P < 0.05）。结论 KIAA0101表达下调介导的SCL-1细胞增殖抑制和侵袭能力降低与p21和基质金属蛋白酶2表达变化相关。 【关键词】 癌，鳞状细胞； 基因，KIAA0101； RNA，小分子干扰

关键词: 癌，鳞状细胞, 基因，KIAA0101, RNA，小分子干扰

Abstract: LI Min, XIA Yong-hua, LIU Dong, FU Dan-dan, LI Zhan-guo, TIAN Zhong-wei. Department of Dermatology, First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan, China Corresponding author: TIAN Zhong-wei, Email: zhonwt@xxmu.edu.cn 【Abstract】 Objective To investigate the effect of downregulation of KIAA0101 protein expression on the proliferation and invasion of a cutaneous squamous cell carcinoma cell line SCL-1, and to explore possible molecular mechanisms underlying the effect. Methods SCL-1 cells were classified into three groups: siRNA control group transfected with the control siRNA, KIAA0101 group transfected with KIAA0101 siRNA, and untreated group remaining untreated. After additional culture, Western blot was used to detect the expression of KIAA0101 protein and proteins associated with cell proliferation and invasion, cell counting kit-8 （CCK-8） to evaluate cellular proliferative activity, and Boyden chamber assay to estimate invasive ability of cells. Results The relative expression level of KIAA0101 protein was 0.062 ± 0.095 in the KIAA0101 group, significantly lower than that in the untreated group （0.359 ± 0.044, P < 0.05） and siRNA control group （0.379 ± 0.025, P < 0.05）. A significant decrease was observed in cellular proliferative activity （from 24 to 96 hours） and invasive activity （at 48 hours） in the KIAA0101 group compared with the other two groups （all P < 0.05）. Moreover, compared with the untreated group and siRNA control group, the KIAA0101 group showed a stronger expression of p21 protein （0.570 ± 0.060 vs. 0.048 ± 0.018 and 0.055 ± 0.014, P < 0.01） but a weaker expression of matrix metalloproteinase 2 （MMP2） protein （0.051 ± 0.013 vs. 0.205 ± 0.029 and 0.221 ± 0.029, P < 0.01）. Conclusion The inhibition of SCL-1 cell proliferation and invasion induced by the downregulation of KIAA0101 gene expression may be associated with the expression changes of p21 and MMP2. 【Key words】 Carcinoma, squamous cell; Genes，KIAA0101; RNA，small interfering

Key words: KIAA0101 gene

中图分类号: 

  • R751