中华皮肤科杂志 ›› 2015, Vol. 48 ›› Issue (11): 774-777.

• 论著 • 上一篇    下一篇

自行制备的HPV6b和11型E7蛋白多克隆抗体检测尖锐湿疣皮损的研究

曹蕾1,程浩2,王惠3,周强4,汤怡5,姜少杰6,丁杨7,陈贤祯8   

  1. 1. 浙江大学医学院；浙江大学医学院附属邵逸夫医院皮肤科；杭州市富阳区第一人民医院皮肤科
    2. 杭州市浙江大学邵逸夫医院皮肤科
    3. 浙江省富阳市人民医院
    4. 浙江大学医学院附属邵逸夫医院
    5. 浙江省人民医院
    6. 浙江大学-医学院-附属邵逸夫医院 生物医学研究中心
    7. 杭州邵逸夫医院皮肤科
    8. 杭州浙江大学医学院附属邵逸夫医院皮肤科
  • 收稿日期:2015-01-07 修回日期:2015-06-21 发布日期:2015-11-03
  • 通讯作者: 程浩 E-mail:hz_chenghao@126.com
  • 基金资助:

    国家自然科学基金面上项目

Detection of human papillomavirus-6b/11 E7 proteins in condyloma acuminatum lesions with home-made polyclonal antibodies

  • Received:2015-01-07 Revised:2015-06-21 Published:2015-11-03

PDF

8

摘要：

目的 采用自行制备的HPV6b和11型E7蛋白多克隆抗体检测外生殖器尖锐湿疣皮损组织中HPV6b和11型E7蛋白的表达，探讨该抗体在临床检验中的应用价值。 方法 55例经临床和病理确诊的尖锐湿疣皮损石蜡标本，用自行制备的HPV6b和11型E7蛋白多克隆抗体进行免疫组化检测，其中18例皮损冰冻标本用RT-PCR法测定HPV6b和11型E7蛋白mRNA表达，分析与免疫组化结果的符合率。 结果 55例尖锐湿疣皮损的免疫组化染色显示，HPV6b和11型E7蛋白为胞核染色，且皮损全层表皮细胞均有阳性表达，但基底层阳性细胞较多。HPV6b和11型E7蛋白阳性表达率分别为76.36%（42/55）和58.18%（32/55），总阳性表达率为94.55%（52/55），两蛋白双阳性率为40.00%（22/55），两蛋白均阴性表达为3例（5.45%）。18例尖锐湿疣冰冻标本经RT-PCR法检测，HPV6b和11型E7 mRNA阳性表达分别为15例和10例，双阳性表达7例，其阳性表达型别与免疫组化结果完全一致，符合率均为100%。 结论 该自行制备的HPV6b和11型E7蛋白多克隆抗体免疫组化法检测尖锐湿疣皮损，检测结果直观，可以观察到HPV6b和11型感染细胞在病损中的分布位置。

Abstract:

Cao Lei, Cheng Hao*, Wang Hui, Zhou Qiang, Tang Yi, Jiang Shaojie, Ding Yang, Chen Xianzhen. *Department of Dermatology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China Corresponding author: Cheng Hao, Email: hz_chenghao@126.com 【Abstract】 Objective To detect the expressions of human papillomavirus （HPV）-6b/11 E7 proteins in condyloma acuminatum lesions in external genitalia with home-made polyclonal antibodies against HPV-6b/11 E7 proteins, and to estimate the clinical application value of the polyclonal antibodies. Methods Fifty-five tissue specimens were collected from the lesions of patients with clinically and pathologically comfirmed condyloma acuminatum, and subjected to paraffin embedding. An immunohistochemical study was conducted to detect the expressions of HPV-6b/11 E7 proteins in the 55 specimens with home-made polyclonal antibodies against HPV-6b/11 E7 proteins. Reverse transcription （RT）-PCR was performed to quantify the mRNA expressions of HPV-6b/11 E7 in 18 frozen specimens from these patients. The coincidence rate between the two methods was analyzed. Results Immunohistochemical staining showed that HPV-6b/11 E7 proteins were expressed in the nuclei of cells throughout the whole epidermis of the lesions, and especially in the basal layer. Of the 55 specimens, 42 （76.36%） stained positive for the HPV-6b E7 protein, 32 （58.18%） for the HPV11 E7 protein, 22 （40.00%） for both of them, 3 （5.45%） for neither of them, with the positive rate of E7 proteins being 94.55% （52/55）. As RT-PCR showed, the HPV-6b and HPV 11 E7 mRNAs were positive in 15 and 10 out of the 18 frozen specimens, respectively, and both positive in 7 specimens. The HPV types identified by RT-PCR in the 15 specimens were fully consistent with those by the immunohistochemical study. Conclusion The immunohistochemical assay using the home-made anti-HPV-6b/11 E7 polyclonal antibodies can be performed to detect condyloma acuminatum lesions and to observe the distribution of HPV-6b/11-infected cells in an intuitionistic way.

中图分类号: 

  • R75

引用本文

曹蕾 程浩 王惠 周强 汤怡 姜少杰 丁杨 陈贤祯. 自行制备的HPV6b和11型E7蛋白多克隆抗体检测尖锐湿疣皮损的研究[J]. 中华皮肤科杂志, 2015,48(11):774-777. doi: