中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (9): 650-653.

• 论著 • 上一篇    下一篇

三种黄芩提取物诱导人皮肤鳞状细胞癌SCL-1细胞凋亡的研究

刘梅1,周春林2,朱红3,金光玉4,陈洪铎5,何春涤1   

  1. 1. 中国医科大学附属第一医院皮肤科
    2. 沈阳中国医科大学第一临床学院皮肤科
    3. 沈阳 中国医科大学附属第一医院皮肤科
    4. 沈阳中国医科大学附属第一医院皮肤科
    5. 中国医科大学第一附属医院皮肤科
  • 收稿日期:2013-09-12 修回日期:2014-04-18 出版日期:2014-09-15 发布日期:2014-09-01
  • 通讯作者: 何春涤 E-mail:chundihe@hotmail.com
  • 基金资助:
    辽宁省科学技术计划;辽宁省教育厅高等学校创新团队项目;辽宁省高等学校优秀人才支持计划

Three Scutellaria baicalensis extracts induce the apoptosis of a human cutaneous squamous cell carcinoma cell line SCL?鄄1: an experimental study

  • Received:2013-09-12 Revised:2014-04-18 Online:2014-09-15 Published:2014-09-01
  • Contact: HE Chun-di E-mail:chundihe@hotmail.com

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摘要： 目的 探讨黄芩苷、黄芩素、汉黄芩素3种黄芩提取物对人皮肤鳞状细胞癌细胞株SCL-1细胞凋亡的影响。 方法 将培养的SCL-1细胞分别用浓度为12.5、25、50、75、100 μmol/L的3种黄芩提取物作用12、24、48 h后，噻唑蓝（MTT）法检测上述药物对SCL-1细胞的生长抑制作用。膜联蛋白V-异硫氰酸荧光素/碘化丙锭双染法和酶联免疫吸附试验（ELISA）检测SCL-1细胞凋亡率。流式细胞仪检测细胞周期。应用线性内差法，测定半数抑制浓度（IC50）；两样本均数比较用Student t检验；多样本均数比较采用方差分析，各样本之间多重比较采用SNK-q检验。 结果 3种黄芩提取物均可抑制SCL-1细胞的生长，并具有浓度及时间依赖性（均P ＜ 0.01）；在相同的药物浓度及作用时间下，黄芩素对SCL-1细胞生长抑制作用最强，黄芩苷抑制作用最弱，汉黄芩素抑制作用介于二者之间，差异具有统计学意义（均P ＜ 0.01）。12.5 μmol/L黄芩苷、黄芩素、汉黄芩素分别作用SCL-1细胞48 h均可诱导SCL-1细胞凋亡并阻滞细胞周期的进程，其中G1期细胞比例分别为56.37% ± 2.41%、74.23% ± 2.02%、64.15% ± 1.87%，早期凋亡率分别为8.09% ± 1.02%、24.13% ± 0.76%、14.45% ± 1.57%，与对照组（45.04% ± 1.93%，4.12% ± 0.29%）比较，差异均有统计学意义（F = 83.29，186.37，均P ＜ 0.01）；其中黄芩素作用最强，其次为汉黄芩素，黄芩苷作用最弱。 结论 黄芩能抑制SCL-1细胞生长并诱导其凋亡，为皮肤鳞状细胞癌的中药治疗开拓新的思路。

关键词: 黄芩, 黄芩苷, 黄芩素, 汉黄芩素, 癌，鳞状细胞, 细胞凋亡

Abstract: Liu Mei, Zhou Chunlin, Zhu Hong, Jin Guangyu, Chen Hongduo, He Chundi. Key Laboratory of Immunodermatology of Ministry of Health; Department of Dermatology, First Affiliated Hospital of China Medical University, Shenyang 110001, China Corresponding author: He Chundi, Email: chundihe@hotmail.com 【Abstract】 Objective To investigate the effect of three Scutellaria baicalensis extracts （baicalin, baicalein and wogonin） on the apoptosis of a human cutaneous squamous cell carcinoma cell line, SCL-1. Methods Methyl thiazolyl tetrazolium （MTT） assay was performed to detect the proliferation of cultured SCL-1 cells treated with different concentrations （12.5, 25, 50, 75 and 100 μmol/L） of baicalin, baicalein and wogonin for various durations （12, 24 and 48 hours）. Some SCL-1 cells were treated with baicalin, baicalein and wogonin of 12.5 μmol/L respectively for 48 hours followed by the detection of cell apoptosis by double staining with annexin V-fluorescein isothiocyanate/propidium iodide in combination with enzyme-linked immunosorbent assay （ELISA）, as well as estimation of cell cycle by flow cytometry. The 50% inhibitory concentration was determined by line regression model and inner insert method, and statistical analysis was carried out by Student′s t test, one-way analysis of variance （ANOVA）, and Student-Newman-Keuls-q test. Results Baicalin, baicalein and wogonin all inhibited the proliferation of SCL-1 cells in a dose- and time-dependent manner （all P ＜ 0.01）. Under the same conditions （treatment concentration and duration）, baicalein showed the strongest inhibitory effect on the proliferation of SCL-1 cells, followed by wogonin and baicalin （all P ＜ 0.01）. All the Scutellaria baicalensis extracts induced the apoptosis of SCL-1 cells and arrested them in G1-phase. The percentage of cells in G1 phase was 56.37% ± 2.41%, 74.23% ± 2.02% and 64.15% ± 1.87%, and early apoptosis rate was 8.09% ± 1.02%, 24.13% ± 0.76% and 14.45% ± 1.57%, in SCL-1 cells treated with baicalin, baicalein and wogonin of 12.5 μmol/L for 48 hours, respectively, compared to 45.04% ± 1.93% and 4.12% ± 0.29% in the untreated control cells respectively （F = 83.29, 186.37, respectively, both P ＜ 0.01）. Similarly, there was a downward trend from baicalein to wogonin and baicalin in the effect on cell apoptosis and cell cycle arrest of SCL-1 cells. Conclusions Scutellaria baicalensis can inhibit the growth and induce the apoptosis of SCL-1 cells, which may provide new ideas for the treatment of cutaneous squamous cell carcinoma with traditional Chinese drugs.

Key words: Scutellaria baicalensis, Baicalin, Baicalein, Wogonin, Carcinoma, squamous cell, Apoptosis