寻常性银屑病患者外周血白介素17、白介素23 mRNA的表达及与病情相关性研究

中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (4): 278-281.

寻常性银屑病患者外周血白介素17、白介素23 mRNA的表达及与病情相关性研究

苏彤¹,陈宏²,李璐³,⁴,门剑龙⁵,李桂珍³

1. 南京明基医院
2. 天津市人民医院皮肤科
3. 天津市中医药研究院附属医院
4.
5. 天津医科大学总医院

收稿日期:2013-07-02 修回日期:2013-08-12 出版日期:2014-04-15 发布日期:2014-04-01
通讯作者: 陈宏 E-mail:baixue20042006@126.com
基金资助:
中华医学会皮肤性病学分会朗生医药有限公司皮肤病学科研基金

Interleukin-17 and interleukin-23 mRNA expressions in peripheral blood of patients with psoriasis vulgaris and their correlations with disease severity

Received:2013-07-02 Revised:2013-08-12 Online:2014-04-15 Published:2014-04-01
Contact: Hong CHEN E-mail:baixue20042006@126.com

摘要/Abstract

摘要： 【摘要】目的探讨白介素17（IL-17）、白介素23（IL-23）mRNA在寻常性银屑病患者与健康人外周血中的表达差异及其与银屑病皮损面积和严重程度指数（PASI）的相关性；探讨白芍总苷在寻常性银屑病治疗中的作用机制。方法采用实时荧光定量PCR方法，检测50例寻常性银屑病患者和40例健康对照者外周血IL-17、IL-23 mRNA的表达量；对42例银屑病患者应用白芍总苷4周后及23例应用8周后外周血IL-17、IL-23 mRNA的表达量进行分析。根据资料性质，利用SPSS16.0软件进行t检验或秩和检验、Pearson相关性分析。结果寻常性银屑病患者外周血中IL-17和IL-23 mRNA的表达水平明显高于健康对照组（两组IL-17 mRNA的ΔCt值分别为-5.32 ± 0.80、2.79 ± 0.76；IL-23 mRNA分别为-5.43 ± 0.68、-3.77 ± 0.86，两组比较，均P < 0.05）；患者外周血IL-17和IL-23 mRNA的表达水平与PASI呈正相关（r值分别为0.61、0.52，均P < 0.05）。经白芍总苷治疗4周后，患者外周血IL-17和IL-23 mRNA的表达及PASI均较治疗前明显降低（IL-17 mRNA的ΔCt值分别为-2.24 ± 0.61、-5.30 ± 0.78；IL-23 mRNA分别为-1.97 ± 0.74、-5.44 ± 0.68；PASI分别为5.8 ± 2.7、9.4 ± 4.2，两组比较，均P < 0.05）；治疗8周后，患者外周血IL-17和IL-23 mRNA的表达及PASI比治疗4周后明显降低（IL-17 mRNA的ΔCt值分别为-1.51 ± 0.78、-2.21 ± 0.59；IL-23 mRNA分别为-1.27 ± 0.81、-1.89 ± 0.72；PASI分别为3.8 ± 1.8、7.3 ± 2.5，两组比较，均P < 0.05）。结论 IL-17和IL-23可能参与了寻常性银屑病的发病过程；应用白芍总苷治疗寻常性银屑病后，IL-17和IL-23 mRNA表达水平下降，且PASI降低。

关键词: 银屑病, 白细胞介素17, 白细胞介素23, 银屑病皮损面积和严重程度指数

Abstract: Su Tong*, Chen Hong, Li Lu, Men Jianlong, Li Guizhen. *Graduate School of Tianjin Medical University, Tianjin 300070, China Corresponding author: Chen Hong, Email: baixue20042006@126.com 【Abstract】 Objective To compare the mRNA expressions of interleukin （IL）-17 and IL-23 in peripheral blood of patients with psoriasis vulgaris versus healthy individuals, assess the relationship of these parameters with psoriasis area and severity index （PASI） score, and to investigate the therapeutic mechanisms of total glucosides of peony（TGP） for psoriasis vulgaris. Methods Fifty patients with psoriasis vulgaris and 40 healthy individuals were enrolled in this study. Of these patients, 42 were treated with TGP of 600 － 900 mg twice a day for 8 weeks. Blood samples were obtained from all the healthy individuals, 50 patients before treatment, 42 patients after 4-week treatment, and 23 patients after 8-week treatment. Real-time fluorescence-based quantitative reverse transcription PCR （RT-PCR） was used to determine the mRNA expression levels of IL-17 and IL-23 in the blood samples. The severity of psoriasis was evaluated using PASI score before and after the treatment. Statistical analysis was done by t test, rank sum test, and Pearson correlation analysis using the SPSS16.0 software. Results The IL-17 and IL-23 mRNA expression levels （given in ΔCt value） in the patients before treatment were significantly higher than those in the healthy controls （IL-17, －5.32 ± 0.80 vs. 2.79 ± 0.76, t = 47.71, P < 0.05; IL-23, －5.43 ± 0.68 vs. －3.77 ± 0.86, t = 10.38, P < 0.05）, and positively correlated with the PASI score （r = 0.61, 0.52 respectively, both P < 0.05）. A significant decrease was observed in the mRNA expression levels of IL-17 and IL-23 as well as PASI score in the 42 patients after 4-week treatment with TGP compared with those before treatment（IL-17, －2.24 ± 0.61 vs. －5.30 ± 0.78, t = 20.40, P < 0.05; IL-23, －1.97 ± 0.74 vs. －5.44 ± 0.68, t = 21.69, P < 0.05; PASI, 5.8 ± 2.7 vs. 9.4 ± 4.2, t = 4.68, P < 0.05）, and in the 23 patients after 8-week treatment compared with those after 4-wek treatment（IL-17, －1.51 ± 0.78 vs. －2.21 ± 0.59, t = 3.50, P < 0.05; IL-23, －1.27 ± 0.81 vs. －1.89 ± 0.72, t = 2.70, P < 0.05; PASI, 3.8 ± 1.8 vs. 7.3 ± 2.5, t = 5.47, P < 0.05）. Conclusions It seems that both IL-17 and IL-23 are involved in the pathogenesis of psoriasis vulgaris, and TGP treatment can reduce the mRNA expression levels of IL-17 and IL-23 as well as PASI score in patients with psoriasis vulgaris.

Key words: Psoriasis, Interleukin-17, Interleukin-23, Psoriasis area and severity index

苏彤陈宏李璐门剑龙李桂珍. 寻常性银屑病患者外周血白介素17、白介素23 mRNA的表达及与病情相关性研究[J]. 中华皮肤科杂志, 2014, 47(4): 278-281.

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