中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (2): 116-119.

• 论著 • 上一篇    下一篇

84株感染相关皮肤病MRSA SCCmec分型及PVL毒素检测

郭利平1,王晓彦2   

  1. 1. 鄂尔多斯市中心医院
    2. 内蒙古医学院附属医院
  • 收稿日期:2013-05-05 修回日期:2013-11-08 出版日期:2014-02-15 发布日期:2014-02-01
  • 通讯作者: 王晓彦 E-mail:xiaoyan4766@hotmail.com

Staphylococcal cassette chromosome mec typing of and detection of PVL gene in 84 methicillin-resistant Staphylococcus aureus isolates from patients with infectious skin diseases

Li-Ping GUO1,   

  • Received:2013-05-05 Revised:2013-11-08 Online:2014-02-15 Published:2014-02-01

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摘要： 【摘要】 目的 探讨感染相关皮肤病耐甲氧西林金黄色葡萄球菌（MRSA）的SCCmec基因型别及PVL毒素携带情况。 方法 分离于感染相关皮肤病MRSA菌株及其相关信息，对所收集到的菌株利用PCR方法进行mecA鉴定，mecA阳性者确定为实验对象，再通过多重PCR的方法进行SCCmec基因分型及PVL毒素的测定。 结果 共收集95株MRSA，经鉴定mecA阳性者为84株，其中有5株携带PVL毒力基因。有69株为SCCmecⅢ型，占86.3%（69/84），有3株为SCCmecⅠ型，占3.6%（3/84），有8株为SCCmecⅣ型，占9.52%（8/84），其余4株为不可分型，占4.8%（4/84）。未发现SCCmecⅡ、Ⅴ两型。 结论 感染相关皮肤病MRSA以HA-MRSA为主，SCCmec分型大部分为Ⅲ型，CA-MRSA及HA-MRSA均可携带PVL毒素。

关键词: 感染, 皮肤, 基因，SCCmec, PVL毒素

Abstract: Guo Liping*, Wang Xiaoyan. *Department of Dermatology, Center Hospital of Erdos City, Erods 017000, Inner Mongolia, China Corresponding author: Wang Xiaoyan, Email: xiaoyan4766@hotmail.com 【Abstract】 Objective To determine staphylococcal cassette chromosome mec （SCCmec） types and detect panton-valentine leukocidin （PVL） gene in methicillin-resistant Staphylococcus aureus （MRSA） isolates from patients with infectious skin diseases. Methods Staphylococcus aureus was isolated from lesion exudate of patients with infectious skin diseases between June 2010 and July 2012 in different departments of Affiliated Hospital of Inner Mongolia Medical University. General and medical information on the patients was collected as well. PCR was performed to detect mecA gene in MRSA strains, and multiplex PCR was conducted to determine SCCmec type and detect PVL gene in mecA gene-positive strains. Results A total of 95 MRSA strains were isolated from these patients with infectious skin diseases, and mecA gene was found in 84 out of these strains. Among these mecA-positive strains, 5 carried PVL gene, 69 （86.3%） were identified as SCCmec type Ⅲ, 3 （3.6%） as SCCmec typeⅠ, 8 （9.52%） as SCCmec type Ⅳ, 4 （4.8%） were non-typeable, and no strain was identified as SCCmec typeⅡ or Ⅴ. Conclusions Hospital-acquired （HA）-MRSA predominates in MRSA strains causing infectious skin diseases, with SCCmec type Ⅲ as the major SCCmec type. Both HA and community-acquired （CA） MRSA strains carry PVL toxin.

Key words: Infection, Skin, Genes, SCCmec, PVL toxin