单细胞RNA测序技术及其在皮肤病学研究中的应用

doi:10.3760/cma.j.issn.0412-4030.2019.07.015

摘要/Abstract

摘要： 【摘要】单细胞RNA测序是指在单细胞水平上对转录组进行测序的方法，是目前生物学研究领域的一项热点技术。与普通高通量RNA测序相比，单细胞RNA测序可以分辨单个细胞间的生物学差异，识别出易被忽视的罕见细胞群体和细胞亚群。本文主要介绍单细胞RNA测序的方法，总结目前该技术在皮肤病研究领域的应用，探讨现有技术的局限性和未来应用前景。

关键词: 序列分析, RNA；单细胞RNA测序；高通量RNA测序；皮肤病学；遗传异质性

Abstract: 【Abstract】 Single-cell RNA sequencing is a method for transcriptome profiling at the single-cell level, and is a hotspot technology in the field of biological research currently. Compared with the common high-throughput RNA sequencing, single-cell RNA sequencing can distinguish the biological differences between single cells, and identify rare cell populations and subpopulations. This review mainly describes the single-cell RNA sequencing method, summarizes current application of this technique in the field of dermatology, and discusses limitations and prospects of this technique.

Key words: Sequence analysis, RNA, Single?cell RNA sequencing, High?throughput RNA sequencing, Dermatology, Genetic heterogeneity

朱容慧李巍. 单细胞RNA测序技术及其在皮肤病学研究中的应用[J]. 中华皮肤科杂志, 2019, 52(7): 506-509.

Zhu Ronghui, Li Wei. Single-cell RNA sequencing and its application in dermatology[J]. Chinese Journal of Dermatology, 2019, 52(7): 506-509.

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