中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (6): 401-403.

• 论著 • 上一篇    下一篇

热处理对体外人黑素细胞及HaCaT细胞分泌α黑素细胞刺激素的影响

祃丽娟1,赵广2,顾伟杰3,牛建荣4,邵丽芳5,孟如松2,闫文厅5,成玉6   

  1. 1. 中国人民解放军空军总医院
    2. 中国人民解放军空军总医院皮肤科 100142
    3. 西安第四军医大学西京医院皮肤科
    4. 空军总医院皮肤科
    5. 解放军空军总医院
    6.
  • 收稿日期:2012-09-17 修回日期:2012-11-05 出版日期:2013-06-15 发布日期:2013-06-01
  • 通讯作者: 赵广 E-mail:guangfen2016@126.com
  • 基金资助:
    国家自然科学基金

In vitro effect of heat treatment on the secretion of α-melanocyte-stimulating hormone by human melanocytes and HaCaT cells

  • Received:2012-09-17 Revised:2012-11-05 Online:2013-06-15 Published:2013-06-01
  • Supported by:
    National Natural Science Foundation of China

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摘要： 【摘要】 目的 探讨热处理后体外培养的人表皮黑素细胞和人角质形成细胞（HaCaT细胞）分泌α黑素细胞刺激素（α-MSH）的变化。方法 将体外培养的黑素细胞及HaCaT细胞分别给予热处理（42 ℃，每天60 min，连续3 d），实时荧光定量PCR法（RT-PCR）及酶联免疫吸附测定法（ELISA）检测两种细胞分泌的α-MSH的变化。结果 黑素细胞热处理组（1.5862 ± 0.3262）与对照组（1.0000 ± 0.2715）相比，α-MSH mRNA表达均明显增加（P < 0.05）；HaCaT细胞热处理组（1.8013 ± 0.1216）与对照组（1.0000 ± 0.2532）相比，mRNA水平也有明显增加（P < 0.05）。ELISA结果：黑素细胞热处理组（0.3142 ± 0.0469）蛋白表达水平明显高于对照组（0.2557 ± 0.0330，P < 0.05）；HaCaT细胞热处理组（0.4632 ± 0.0345）蛋白水平也明显高于对照组（0.4389 ± 0.0399，P < 0.05）。结论 热处理可促进人黑素细胞及HaCaT细胞α-MSH分泌。 【关键词】 热； 黑素细胞； HaCaT细胞； α促黑素

关键词: 黑素细胞, HaCaT细胞, α促黑素, 热

Abstract: MA Li-juan, ZHAO Guang, GU Wei-jie, NIU Jian-rong, SHAO Li-fang, MENG Ru-song, YAN Wen-ting, CHENG Yu. Air Force General Hospital of PLA，Beijing 100142, China Corresponding author: ZHAO Guang, Email: guangfen@ yahoo.com 【Abstract】 Objective To evaluate the in vitro effect of heat treatment on the secretion of α-melanocyte-stimulating hormone （α-MSH） by human melanocytes and HaCaT keratinocytes. Methods Cultured normal human melanocytes from forskin tissue and HaCaT cells were treated with hyperthermia at 42 ℃ for 1 hour per day for 3 days. Subsequently, real time fluorescence-based quantitative PCR and enzyme-linked immunosorbent assay （ELISA） were performed to detect the mRNA and protein expressions of α-MSH, respectively. Results The mRNA expression level of α-MSH was significantly higher in heat-treated melanocytes and HaCaT cells than in those untreated （1.5862 ± 0.3262 vs. 1.0000 ± 0.2715, P < 0.05; 1.8013 ± 0.1216 vs. 1.0000 ± 0.2532, P < 0.05）. Moreover, ELISA showed a statistical increase in the supernatant level of α-MSH protein in heat-treated melanocytes and HaCaT cells compared with those untreated （0.3142 ± 0.0469 vs. 0.2557 ± 0.0330, P < 0.05; 0.4632 ± 0.0345 vs. 0.4389 ± 0.0399, P < 0.05）. Conclusion Heat treatment can promote the secretion of α-MSH by melanocytes and HaCaT cells. 【Key words】 Heat; Melanocytes; HaCaT cells; α-MSH