中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (2): 84-87.

• 论著 • 上一篇    下一篇

姜黄素对肿瘤坏死因子-α诱导的HaCaT细胞增殖及凋亡的影响

杨晓红1,曹毅2,刘改荣3,代群1,解凡1,李园园1,陈微1   

  1. 1. 浙江省中医院
    2. 浙江中医药大学第一附属医院
    3. 浙江中医药大学
  • 收稿日期:2012-02-21 修回日期:2012-04-06 出版日期:2013-02-15 发布日期:2013-02-01
  • 通讯作者: 曹毅 E-mail:caoyi1965@163.com
  • 基金资助:
    Jagged1/Notch1信号系统在银屑病发病机制中作用及其调控研究

Effect of curcumin on the proliferation of and apoptosis in HaCaT cells induced by tumor necrosis factor α

  • Received:2012-02-21 Revised:2012-04-06 Online:2013-02-15 Published:2013-02-01

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摘要： 目的 探讨姜黄素对肿瘤坏死因子（TNF）-α诱导的HaCaT细胞增殖及凋亡的影响。方法 MTT法检测不同浓度重组TNF-α（0、1、5、10、25、50、100 ng/ml）、姜黄素20 μmol/L对HaCaT细胞增殖的影响。蛋白印迹实验检测25 ng/ml TNF-α和20 μmol/L姜黄素作用下HaCaT细胞PCNA、 Notch-1 的蛋白表达。使用Annexin-V/PI试剂盒在流式细胞仪上检测25 ng/ml TNF-α和20 μmol/L姜黄素作用下HaCaT细胞早期凋亡情况。结果 TNF-α呈浓度依赖性促进HaCaT细胞增殖，25 ng/ml TNF-α对HaCaT细胞的促增殖基本达到最大，20 μmol/L姜黄素有效抑制25 ng/ml TNF-α对HaCaT细胞的促增殖作用，25 ng/ml TNF-α对HaCaT细胞无明显促凋亡作用，而20 μmol/L姜黄素有效促进HaCaT细胞发生凋亡，并可激发25 ng/ml TNF-α对HaCaT细胞的凋亡诱导作用。 结论 姜黄素可激发TNF-α对HaCaT细胞的促凋亡作用，同时抑制TNF-α对HaCaT细胞促增殖作用。

关键词: 银屑病, 肿瘤坏死因子α, 姜黄素

Abstract: YANG Xiao-hong, CAO Yi, LIU Gai-rong, DAI Qun, XIE Fan, LI Yuan-yuan, CHEN Wei. First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou 310006, China Corresponding author: CAO Yi, Email: caoyi1965@163.com 【Abstract】 Objective To evaluate the effect of curcumin on the proliferation of and apoptosis in HaCaT cells induced by tumor necrosis factor α （TNF-α）. Methods HaCaT cells were cultured with the presence of different concentrations（0, 1, 5, 10, 25, 50, 100 ng/ml） of recombinant TNF-α, curcumin of 20 μmol/L, or the combination of recombinant TNF-α （25 ng/ml） and curcumin （20 μmol/L）, for 24 hours followed by the determination of cell proliferation with methyl thiazolyl tetrazolium （MTT） assay. Western blot was conducted to measure the protein expression of proliferating cell nuclear antigen （PCNA） and Notch-1 in HaCaT cells treated with recombinant TNF-α（25 ng/ml） and curcumin（20 μmol/L） alone or in combination for 24 hours. Flow cytometry using annexin-V/propidium iodine （PI） was performed to assess the early apoptosis in HaCaT cells incubated with recombinant TNF-α of 25 ng/ml and curcumin of 20 μmol/L alone or in combination for 12 hours. Statistical analysis was carried out with one-way analysis of variance. Results Recombinant TNF-α promoted the proliferation of HaCaT cells in a dose-dependent manner, with the maximum proliferation activity observed in HaCaT cells treated with TNF-α of 25 ng/ml, while curcumin of 20 μmol/L effectively inhibited the proliferation of HaCaT cells induced by TNF-α of 25 ng/ml （P < 0.01）. TNF-α of 25 ng/ml had no obvious effect on cell apoptosis, while curcumin of 20 μmol/L markedly induced the apoptosis in HaCaT cells, and there was a synergy between TNF-α of 25 ng/ml and curcumin of 20 μmol/L in the induction of apoptosis in HaCaT cells, with the apoptosis rate being 2.3%, 3.4%, 11.6% and 16.8% respectively in untreated cells, cells treated with TNF-α, curcumin, and the combination of TNF-α and curcumin, respectively. Conclusions Curcumin could enhance the inductive effect of TNF-α on the apoptosis in, but suppress the promotive effect of TNF-α on the proliferation of, HaCaT cells. 【Key words】 Psoriasis; Tumor necrosis factor-alpha; Curcumin

Key words: TNF-α, curcumin

中图分类号: 

  • R751