中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (9): 606-609.

• 论著 • 上一篇    下一篇

不同病期银屑病皮损组织CK20、S100A7、SP的表达及其相关性

刘太华1,刘德芳2,陈易华2,等3   

  1. 1. 成都军区总医院
    2.
    3. 北京首都医科大学100069
  • 收稿日期:2010-01-04 修回日期:2010-03-08 发布日期:2010-09-10
  • 通讯作者: 刘太华 E-mail:thliu1966@yahoo.cn

Expressions of CK20, S100A7 and substance P in different stages of psoriatic lesions and their relationship

  • Received:2010-01-04 Revised:2010-03-08 Published:2010-09-10

摘要:

目的 探讨不同病期银屑病皮损组织中CK20、S100A7、SP的表达及CK20与S100A7、SP表达的关系。方法 选择经过非正规治疗,新旧皮损同时存在的患者19例,获取皮损旁的正常皮肤组织(发病前期)、皮损组织(进展期)和皮损修复后的病灶皮肤组织(缓解期),观察皮损中免疫组化CK20、S100A7、SP的表达情况。结果 免疫组化图像分析各组A值,发病前期组、进展期组和缓解组CK20分别为7683.80 ± 6134.55、18305.04 ± 13171.30、7257.53 ± 4417.75。S100A7分别为8789.05 ± 6240.91、18058.01 ± 16537.18、9295.65 ± 9310.02。SP分别为3242.51 ± 3775.41、9364.98 ± 7596.64、2910.85 ± 3349.46。进展期皮损组织中CK20与S100A7,SP表达相对于发病前期和缓解期明显增加 其差异均有统计学意义,P < 0.05。发病前期组与缓解组其差异均无统计学意义,P > 0.05。CK20与S100A7、SP表达呈正相关,相关系数R分别为0.779、0.876、P < 0.05。结论 银屑病发病与Merkel细胞数量的变化有一定的关系。

关键词: 银屑病, Merkel细胞, CK20, S100A7, SP, CD13

Abstract:

Objective To explore the expressions of CK20, S100A7 and substance P (SP) in different stages of psoriatic lesions and their relationship. Methods A total of 19 patients, who had received irregular treatment for psoriasis and had both progressive and healed psoriatic lesions, were enrolled in this study. Skin tissue specimens were obtained from perilesional normal skin, progressive lesions and healed lesions of these patients and subjected to immunohistochemical analysis of expressions of CK20, S100A7 and SP. Results The relative expression level (absorbance value obtained from immunohistochemical analysis) was 7683.80 ± 6134.55, 18305.04 ± 13171.30, 7257.53 ± 4417.75 for CK20, 8789.05 ± 6240.91, 18058.01 ± 16537.18, 9295.65 ± 9310.02 for S100A7, 3242.51 ± 3775.41, 9364.98 ± 7596.64, 2910.85 ± 3349.46 for SP, respectively, in normal skin, progressive lesions and healed psoriatic lesions. A significant increase was observed in the expressions of CK20, S100A7 and SP in progressive lesions compared with normal skin and healed lesions, whereas no statistical difference was noted in those between normal skin and healed lesions (P > 0.05). The expression of CK20 was positively correlated with that of S100A7 and SP (r = 0.779, 0.876, both P < 0.05). Conclusion The pathogenesis of psoriasis seems to be associated with the changes in the number of Merkel cells.

Key words: Psoriasis, Merkel cell, CK20, S100A7, SP, CD13