中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (2): 108-110.

• 论著 • 上一篇    下一篇

银屑病骨髓间质干细胞的生物学特性

孟维维1,田畔2,张振颖3,刘晓明4   

  1. 1. 大连医科大学附属第一医院
    2.
    3. 大连医科大学附属第一医院皮肤科教研室
    4. 大连医科大学附属第一医院皮肤科
  • 收稿日期:2009-03-16 修回日期:2009-05-06 出版日期:2010-02-15 发布日期:2012-03-30
  • 通讯作者: 刘晓明 E-mail:Lxm_1956@yahoo.com.cn
  • 基金资助:
    30872271;国家自然科学基金资助项目（编号）

Biological characteristics of bone mesenchymal stem cells from psoriatic patients

  • Received:2009-03-16 Revised:2009-05-06 Online:2010-02-15 Published:2012-03-30
  • Contact: liu xiaoming E-mail:Lxm_1956@yahoo.com.cn

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摘要： 目的 探讨银屑病骨髓间质干细胞生长情况及生物学特性。方法 密度梯度离心法分离培养骨髓单一核细胞，贴壁纯化法得到骨髓间质干细胞，镜下观察细胞形态，密度，生长情况。流式细胞仪鉴定细胞表面抗原，MTT法绘出生长曲线。结果 正常和银屑病患者原代细胞在形态学上差异无统计学意义，原代培养时银屑病组骨髓细胞贴壁晚，融合速度慢，培养时间长。传代后细胞形态无差异。经鉴定两者细胞表面CD29表达均为阳性，CD34，CD45表达均为阴性；生长曲线银屑病患者PI代第4天开始出现较低的增殖活性，A470为0.081 ± 0.0066，对照组A470为0.095 ± 0.0130，t = 2.358，P < 0.05。结论 银屑病患者骨髓间质干细胞原代增值活性较低，细胞形态与对照组差异无统计学意义。

关键词: 银屑病, 间质干细胞, 生物学

Abstract: Objective To observe the growth and biological features of bone mesenchymal stem cells （BMSC） from psoriatic patients. Methods Peripheral blood mononuclear cells were isolated from 5 patients with active psoriasis vulgaris and 5 normal human controls, and BMSC were obtained and purified using plastic adherence method followed by primary culture and passage in vitro. The cell morphology, density and growth were observed with microscopy. Cell growth pattern was evaluated by MTT assay. Flow cytometry was applied to identify surface antigens, including CD29, CD34, CD45 and CD106, on these cells. Results No significant difference was observed in the morphology of primary or descendant BMSC between the patients and controls. The primary BMSC from psoriatic patients tended to adhere to the plastic wall later, confluence and grow more slowly compared with those from the controls. The BMSCs from both psoriatic patients and healthy donors were positive for CD29, but negative for CD34 or CD45. On the 4th day of culture, the BMSC from psoriatic patients exhibited a decrease in proliferation, with the absorbence at 470 nm （A470） being 0.081 ± 0.0066 and 0.095 ± 0.0130, respectively for BMSC from the patients and controls （t = 2.358, P < 0.05）. Conclusion There is a decrease in the proliferation of BMSC from psoriatic patients which show a morphological similarity to those from healthy controls.