中华皮肤科杂志 ›› 2009, Vol. 42 ›› Issue (11): 760-762.

• 论著 • 上一篇    下一篇

甲氨蝶呤对寻常性银屑病患者外周血CD14+单核细胞Toll样受体2，4表达的影响

罗权1,林玲1,李嘉彦2,张锡宝1,等3   

  1. 1. 广州市皮肤病防治所
    2.
    3. 北京首都医科大学100069
  • 收稿日期:2008-10-08 修回日期:2009-06-24 出版日期:2009-11-15 发布日期:2009-11-13
  • 通讯作者: 罗权 E-mail:luoquan666@126.com
  • 基金资助:

    07001961;省级基金（编号）

Affection of MTX on the expression of TLR2、TLR4 in human peripheral blood mononuclear cell of patients with psoriasis vulgaris

  • Received:2008-10-08 Revised:2009-06-24 Online:2009-11-15 Published:2009-11-13

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摘要：

【摘要】目的 研究TLR2、TLR4在寻常性银屑病发病中的作用及甲氨蝶呤（MTX）对其表达的影响，探讨MTX治疗寻常性银屑病的机制。方法 采用流式细胞术检测43例寻常性银屑病患者经MTX治疗前、治疗4周后、治疗8周后外周血CD14+单核细胞TLR2、TLR4的表达情况，另检测了30例正常人外周血CD14+单核细胞TLR2、TLR4表达情况作为对照组。结果 寻常性银屑病患者外周血中CD14+单核细胞TLR2、TLR4的表达较正常人对照组明显增高（P<0.01），而进行期的寻常性银屑病患者外周血中CD14+单核细胞TLR2、TLR4的表达高于静止期的患者的表达（P<0.05）。经MTX治疗后寻常性银屑病患者外周血中CD14+单核细胞TLR2、TLR4的表达明显低于治疗前（P<0.05）。TLR2和TLR4的表达率与银屑病PASI评分无明显相关性(P﹥0.05)。结论TLR2、TLR4及其介导的天然免疫应答在银屑病发病机制中起重要作用，而MTX可能通过抑制TLR2、TLR4表达而发挥其作用，这有可能是MTX治疗银屑病的作用机理之一。

关键词: 银屑病, Toll样受体, 甲氨蝶呤

Abstract:

【Abstract】Objective To investigate the regulation of MTX on the expression of TLR2、TLR4 in human peripheral blood mononuclear cell of patients with psoriasis vulgaris and explore the treatment mechanism. Methods The Positive rates of TLR2 and TLR4 in human leucocytes were measured by flow cytometry in patients with psoriasis vulgaris before and after treatment with MTX. Results The Positive rates of TLR2 and TLR4 in human leucocytes were significant increase in patients with psoriasis vulgaris as compared with normal controls (P<0.01), and there was significant difference between the active stage of psoriasis vulgaris and the resting stage(P<0.05). There was significant difference between before and after treatment with MTX (P<0.01). The expression of TLR2 and TLR4 was not correlated with PASI scores(P>0.05). Conclusions The TLR2 and TLR4 and the innate immune response mediated by it may be very important to the pathogenesis of psoriasis vulgaris. It is indicated that inhibiting the expression of TLR2、TLR4 may be involved in the treatment of psoriasis vulgaris with MTX.

Key words: psoriasis