中华皮肤科杂志 ›› 2018, Vol. 51 ›› Issue (8): 607-608.doi: 10.3760/cma.j.issn.0412-4030.2018.08.013

• 研究报道 • 上一篇    下一篇

寻常性银屑病患者表皮中DNA甲基化转移酶2和3a mRNA的表达

毛旭华1,栾超2,胡煜3,蒋明军4,陈敏4   

  1. 1. 江苏省宜兴市人民医院
    2. 中国医学科学院南京皮肤病研究所
    3. 南京，中国医学科学院北京协和医学院皮肤病研究所
    4. 南京 中国医学科学院北京协和医学院皮肤病研究所
  • 收稿日期:2017-06-05 修回日期:2017-08-15 出版日期:2018-08-15 发布日期:2018-07-31
  • 通讯作者: 陈敏 E-mail:drchenmin@126.com
  • 基金资助:
    江苏省自然科学基金;人力资源和社会保障部留学人员科技活动项目择优资助项目;中国医学科学院医学与健康科技创新工程项目

mRNA of DNA methyltransferase 2 and 3a in the epidermis of patients with psoriasis vulgaris

Mao Xuhua, Luan Chao, Hu Yu, Dong Huiyan, Jiang Mingjun, Chen Min   

  1. Clinical Laboratory, Yixing People′s Hospital, Yixing 214200, Jiangsu, China （Mao XH）; Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China （Luan C, Hu Y, Jiang MJ, Chen M）; Xuzhou Medical University, Xuzhou 221004, China （Dong HY）
  • Received:2017-06-05 Revised:2017-08-15 Online:2018-08-15 Published:2018-07-31
  • Contact: Chen Min E-mail:drchenmin@126.com
  • Supported by:
    Natural Science Foundation of Jiangsu Province of China;CAMS Innovation Fund for Medical Sciences

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摘要： 目的 检测寻常性银屑病患者表皮中DNA甲基化转移酶（DNMT2和DNMT3a）mRNA表达。方法 利用实时定量PCR技术检测2009年3月至2010年12月来自中国医学科学院皮肤病医院皮肤科及宜兴市人民医院皮肤科门诊的46例寻常性银屑病患者皮损和非皮损表皮以及来自中国医学科学院皮肤病医院皮肤外科的28例健康对照表皮中DNMT2和DNMT3a mRNA的表达。结果 在银屑病患者皮损、非皮损和对照组表皮DNMT2 mRNA表达水平（2-ΔΔCt值）分别是0.62 ± 0.02、0.36 ± 0.05和0.15 ± 0.11，皮损组明显高于非皮损组（t = 6.23，P < 0.01），非皮损组明显高于对照组（t = 7.33，P < 0.01）；DNMT3a mRNA表达水平（2-ΔΔCt值）分别是0.85 ± 0.03、0.43 ± 0.04和0.18 ± 0.09，皮损组明显高于非皮损组（t = 5.66，P < 0.01），非皮损组明显高于对照组（t = 8.62，P < 0.01）。结论 寻常性银屑病患者表皮DNMT2和DNMT3a mRNA均异常高表达。

关键词: 银屑病, DNA修饰甲基酶类, DNA甲基转移酶2, DNA甲基转移酶3a

Abstract: Mao Xuhua, Luan Chao, Hu Yu, Dong Huiyan, Jiang Mingjun, Chen Min Clinical Laboratory, Yixing People′s Hospital, Yixing 214200, Jiangsu, China （Mao XH）; Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China （Luan C, Hu Y, Jiang MJ, Chen M）; Xuzhou Medical University, Xuzhou 221004, China （Dong HY） Corresponding author: Chen Min, Email: drchenmin@126.com 【Abstract】 Objective To investigate the of DNA methyltransferase 2 （DNMT2） and 3a （DNMT3a） in the epidermis of patients with psoriasis vulgaris. Methods Between March 2009 and December 2010, 46 patients with psoriasis vulgaris were enrolled from the Department of Dermatology, Hospital for Skin Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, and the Department of Dermatology of Yixing People′s Hospital, and 28 healthy controls were enrolled from the Department of Dermatologic Surgery, Hospital for Skin Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College. Real-time quantitative PCR was performed to determine the mRNA of DNMT2 and DNMT3a in the epidermis of the lesional and nonlesional skin of patients with psoriasis vulgaris, as well as in the epidermis of normal skin of the healthy controls. Results The mRNA of DNMT2 （expressed as 2-△△Ct） in the lesional skin, non-lesional skin of the patients and normal skin of the healthy controls was 0.62 ± 0.02, 0.36 ± 0.05 and 0.15 ± 0.11, respectively. The mRNA of DNMT2 was significantly higher in the lesional skin than in the non-lesional skin of the patients （t = 6.23, P < 0.01）, and higher in the non-lesional skin of the patients than in the normal skin of the healthy controls （t = 7.33, P < 0.01）. Additionally, the mRNA of DNMT3a was significantly higher in the lesional skin （0.85 ± 0.03） than in the non-lesional skin （0.43 ± 0.04） of the patients （t = 5.66, P < 0.01）, and higher in the non-lesional skin of the patients than in the normal skin of healthy controls （0.18 ± 0.09, t = 8.62, P < 0.01）. Conclusion Both DNMT2 and DNMT3a mRNA were abnormally expressed in the epidermis of patients with psoriasis vulgaris.

Key words: Psoriasis, DNA modification methylases, DNMT2, DNMT3a