中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (8): 550-551.

• 短篇论著 • 上一篇    下一篇

N-乙酰氨基葡萄糖对皮肤角质形成细胞透明质酸合成的影响

张荣鑫 涂彩霞 白明辉 吴茉莉 黄畋   

  1. 大连医科大学附属第二医院皮肤科 大连医科大学附属第二医院皮肤科 大连医科大学附属第二医院皮肤科 大连医科大学 大连医科大学附属第二医院皮肤科
  • 收稿日期:2007-09-10 修回日期:2008-04-20 发布日期:2008-08-15
  • 通讯作者: 张荣鑫 E-mail:zrx_1980@126.com

Effects of N-acetylglucosamine on hyaluronic acid synthesis in human epidermal keratinocytes

Rong-Xin ZHANG Ming-Hui BAI Mo-Li WU   

  • Received:2007-09-10 Revised:2008-04-20 Published:2008-08-15
  • Contact: Rong-Xin ZHANG E-mail:zrx_1980@126.com

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摘要： [摘要] 目的 研究外源性N-乙酰氨基葡萄糖（NAG）对体外培养的人皮肤角质形成细胞（HEKa）透明质酸（HA）合成的影响。方法 采用MTT法检测不同浓度NAG对HEKa细胞增殖的影响；ELISA方法检测不同浓度NAG对HEKa细胞HA合成的影响；RT-PCR法检测NAG对HEKa细胞透明质酸合成酶（即HAS1、HAS2和HAS3）mRNA表达的影响。结果MTT结果表明，1mM/L、3mM/L和5mM/L的NAG对HEKa细胞的增殖无明显影响（P>0.05），而10 mM/L的NAG显著抑制HEKa细胞的增殖（P<0.05）；ELISA 结果显示，NAG呈剂量依赖性促进HEKa细胞HA的生成（P<0.01），最佳浓度为5mM/L；RT-PCR结果显示，NAG对细胞HAS1、HAS2和HAS3mRNA的表达无明显促进作用。结论 外源性NAG具有促进体外培养的HEKa细胞合成HA的作用，而对HAS mRNA的表达无明显影响。

关键词: N-乙酰氨基葡萄糖, 角质形成细胞, 透明质酸, 透明质酸合成酶

Abstract: [Abstract] objective To study the effects of exogenous N-acetylglucosamine (NAG) on hyaluronic acid (HA) production in human epidermal keratinocytes （HEKa）. Methods The cell proliferation、HA production and the expression of hyaluronic acid synthetase (HAS1、HAS2 and HAS3 )mRNA affected by NAG in HEKa cells were separately measured by MTT、ELISA and RT-PCR methods. Results The result of MTT indicated that NAG at 1mM/L、3mM/L and 5mM/L had no effect on cell proliferation of HEKa cells (P>0.05), but NAG at 10 mM/L significantly inhibited the cell proliferation (P<0.05)；The results of ELISA showed that NAG promoting HA production in HEKa cells in dose dependence（P<0.01），and the best concentration was 5mM/L. The results of RT-PCR showed that NAG had no effect on the expression of HAS1、HAS2 and HAS3 mRNA in HEKa cells. Conclusions Exogenous NAG had promoting effect on HA synthesis of HEKa, but no effect on expression of HAS mRNA.

Key words: N-Acetylglucosamine, Human epidermal keratinocytes, Hyaluronic acid, Hyaluronic acid synthetase