中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (8): 552-553.

• 短篇论著 • 上一篇    下一篇

多发性红色毛癣菌病患者不同部位菌株的分子鉴定

青春 冉玉平 李发增 代亚玲 赵国庆 刘素玲 何晓丹 周光平   

  1. 四川省绵阳市科学城医院 成都华西医科大学第一附属医院皮肤科 成都市四川大学华西医院皮肤性病科 成都市四川大学华西医院皮肤科
  • 收稿日期:2008-01-17 修回日期:2008-04-14 发布日期:2008-08-15
  • 通讯作者: 青春 E-mail:qingchun78@126.com

Molecular identification of Trichophyton rubrum strains isolated from multiple body sites of one patient

  

  • Received:2008-01-17 Revised:2008-04-14 Published:2008-08-15

PDF

100

摘要： 目的 用形态学、生物化学和分子生物学方法鉴定和分析从一例多发性皮肤癣菌病患者6个患病部位分离的菌种和菌株相关性。方法 6个部位的分离株先用形态学和生化方法鉴定，再用PCR扩增ITS（内转录间隔区）经序列分析证实全为红色毛癣菌。利用红色毛癣菌特异的随机扩增多态性（RAPD）和串联重复亚单位1和2（Trs-1/Trs-2）的PCR扩增产物判断6菌株基因结构的异同。结果 RAPD可将6株红色毛癣菌分为5个带型。Trs-1扩增产物可将6株菌分为3个带型，并能区分RAPD未能区分的菌株。结论 用RAPD、Trs-1/Trs-2的PCR扩增发现此多发性红色毛癣菌病患者6个感染部位的菌株均不相同，提示同一患者感染的多源性和多克隆性。

关键词: 红色毛癣菌, 分子生物学, 鉴定

Abstract: Objective We report a case of patient with six-lesion of Trichophytosis, the isolate from each lesion was identified to species and strain level, and their relationship was analyzed by means of the morphological, biochemical and molecular biological methods. Methods Primarily, the 6 strains were identified by morphological and biochemical methods, then the internal transcribed spacer (ITS) products was sequenced after PCR, the results indicated that all of isolates were Trichophyton rubrum. Then T.rubrum specialized RAPD and Trs-1/Trs-2 amplification were conducted to differentiate the 6 strains. Results There are 5 different strain types according to RAPD while 3 different strain types in Trs-1 result, which could differentiate the strains that RAPD could not does. Conclusions All of the six-lesions were infected different strains of T. rubrum by combination of RAPD, Trs-1/Trs-2 PCR application, indicating that the multiple origins and colonies of the same one patient with Trichophytosis rubrum.

Key words: Trichophyton rubrum, molecular biology, identification