中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (6): 400-402.

• 论著 • 上一篇    下一篇

他克莫司对人角质形成细胞增殖及干细胞因子分泌的影响

杜娟 樊春红 何培英 张建中   

  1. 北京大学人民医院皮肤科 北京大学人民医院皮肤科 北京大学人民医院皮肤科
  • 收稿日期:2007-09-24 修回日期:2008-03-31 发布日期:2008-06-15
  • 通讯作者: 杜娟 E-mail:dujuan223@sohu.com;dujuan223@medmail.com.cn

Effects of tacrolimus on cell proliferation of and secretion of stem cell factors by cultured human keratinocytes

  

  • Received:2007-09-24 Revised:2008-03-31 Published:2008-06-15

PDF

112

摘要： 目的 探讨他克莫司对人角质形成细胞的增殖及干细胞因子（SCF）分泌的影响。方法 体外HaCaT细胞（人永生化角质形成细胞）培养，用MTT和ELISA分别测定他克莫司对HaCaT细胞增殖及SCF含量的影响。结果 103 nmol/L ~ 104 nmol/L的他克莫司对人HaCaT细胞增殖有抑制作用。10 nmol/L ~ 102 nmol/L的他克莫司可以促进HaCaT细胞SCF分泌量的增加；103 nmol/L的他克莫司对HaCaT细胞SCF的分泌无影响；高浓度104 nmol/L的他克莫司则可抑制HaCaT细胞SCF的分泌。结论 适当浓度的他克莫司能够促进角质形成细胞SCF的分泌。

关键词: Fk506, 白癜风, 黑素细胞, 他克莫司

Abstract: Objective To study the effects of tacrolimus on the cell proliferation of and secretion of stem cell factors（SCF） by cultured human keratinocytes. Methods Human keratinocyte cell line HaCaT was cultured and treated with various concentrations （0, 10, 102, 103, 104 nmol/L） of tacrolimus. After 48 hours of treatment, cell proliferation was measured with MTT assay , the levels of stem cell factor in the culture supernatant of HaCaT cells were detected with ELISA. Results Inhibited proliferation was observed in HaCaT cells treated with tacrolimus of 103-104 nmol/L. The secretion of SCF by HaCaT cells was enhanced in the presence of 10-102 nmol/L of tacrolimus, was inhibited in the presence of tacrolimus of 104 nmol/L, and remained unchanged with 103 nmol/L. Conclusion Certain concentrations of tacrolimus could enhance the expression of SCF in HaCaT cells, which may be associated with the efficacy of tacrolimus in the treatment of vitiligo.

Key words: FK506, Vitiligo, Melanocyte, Tacrolimus