中华皮肤科杂志

• 论著 •    下一篇

银屑病患者CD147、亲环素A和亲环素B表达

陈翔, 林伟, 林静, 粟娟, 吴轶西, 李娟, 常静   

  1. 中南大学湘雅医院皮肤科, 长沙 410008
  • 收稿日期:2005-12-15 出版日期:2006-04-15 发布日期:2006-04-15
  • 基金资助:
    国家自然科学基金资助项目(30200248,30571682);霍英东高校青年教师基金(91040);教育部优秀青年教帅资助计划(2003-189)

Expression of CD147,cyclophilin A and cyclophilin B in patients with psoriasis

CHEN Xiang, LIN wei, LIN Jing, SU Juan, WU YI-xi, LI Juan, CHANG Jing   

  1. Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, China
  • Received:2005-12-15 Online:2006-04-15 Published:2006-04-15

PDF (PC)

115

赞

摘要： 目的 探讨CD147、亲环素A和亲环素B存银屑病发病机制中的作用.方法 用RT-PCR半定量检测40例寻常性银屑病、15例脓疱性银屑病患者和20例正常人外周血T细胞和中性粒细胞中CD147、亲环素A和亲环素B mRNA的表达水平.结果 在T细胞中,寻常性银屑病组CD147、亲环素A和亲环素B mRNA表达水平分别为1.231±0.128、1.254±0.096和1.667±0.166,显著高于正常人对照组(分别为0.983±0.105、1.084±0.070和1.386±0.152,P值均<0.01),且患者组三者表达水平均与其相应的PASI积分呈正相关(r值分别为0.544、0.643和0.668,P值均<0.01);脓疱性银屑病组三者表达水平分别为1.418±0.117、1.760±0.160和1.959±0.156,显著高于寻常性组(P值均<0.01).寻常性银屑病组中性粒细胞CD147 mRNA表达水平(2.118±0.278)显著高于正常人对照组(1.393±0.144,P<0.01),且与其相应的PASI积分呈正相关(r值为0.618,P<0.01);脓疱性银屑病组中性粒细胞CD147的表达水平(3.072±0.371)显著高于寻常性银屑病组(P<0.01).寻常性银屑病组亲环素A和亲环素B mRNA表达水平分别为1.127±0.086和1.081±0.124,与正常人对照组比较差异无统计学意义(P值均>0.05);并与其相应的PASI积分无线性相关关系(r值分别为0.290和0.144,P值均>0.05).脓疱性银屑病组二者的表达水平(分别为1.116±0.075和1.096±0.133)与寻常性银倩病组比较差异无统计学意义(P值均>0.05).结论 CD147、亲环素A和亲环素B可能在银屑病疾病活动和发展过程中起作用.

关键词: 银屑病, 抗原,CD147, 亲环素A, 亲环素B

Abstract: Objective To investigate the roles of CD147,cyclophilin A (CyPA) and cyclophilin B (CyPB) in the pathogenesis of psoriasis.Methods Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to semi-quantitatively measure the mRNA expression of CD147,CyPA and CyPB in peripheral blood T cells and neutrophils from 40 patients with psoriasis vulgaris,15 patients with psoriasis pustulosa and 20 normal controls.Results The mRNA expression levels of CD147,CyPA and CyPB in T cells from patients with psoriasis vulgaris were significantly higher than those from normal controls (1.231±0.128 vs 0.983±0.105,1.254±0.096 vs 1.084±0.070,1.667±0.166 vs 1.386±0.152,respectively,all P<0.01),but significantly lower than those from patients with psoriasis pustulosa (1.231±0.127 vs 1.418±0.117,1.254±0.095 vs 1.760±0.160,1.667±0.165 vs 1.959±0.156,respectively,all P<0.01),and they were positively correlated with the corresponding psoriasis area and severity indix (PASI) score (r=0.544,0.643,0.668 respectively,all P<0.01).The mRNA expression level of CD147 in neutrophils from patients with psoriasis vulgaris was significantly higher than that from normal controls (2.118±0.278 vs 1.393±0.144,P<0.01),but lower than that from patients with psoriasis pustulosa (2.118±0.278 vs 3.072±0.371,P<0.01),and it also positively correlated with the corresponding PASI score (r=0.618,P<0.01).There was no statistical difference between the mRNA expression levels of CyPA and CyPB in neutrophils from the patients with psoriasis vulgaris and those from the normal controls (1.127±0.086 vs 1.253±0.111,1.081±0.124 vs 1.067±0.134 respectively,both P>0.05) or those from the patients with psoriasis pustulosa (1.127±0.086 vs 1.116±0.075,1.081±0.124 vs 1.096±0.133 respectively,both P>0.05),and no correlation was found between them and the corresponding PAS1 score (r=0.290 and 0.144 respectively,both P>0.05).Conclusion CD147,CyPA and CyPB might play a role in the disease activity of and progress in psoriasis.

Key words: Psoriasis, Antigens,CD147, Cyclophilin A, Cyclophilin B