中华皮肤科杂志

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生殖道沙眼衣原体对几种抗菌药物敏感性的研究

韩建德, 陈木开, 廖绮曼, 王莉红, 凌宏忠, 涂裕英   

  1. 中山大学附属第一医院皮肤科, 广州 510080
  • 收稿日期:2006-02-22 出版日期:2006-04-15 发布日期:2006-04-15
  • 基金资助:
    广东省科技计划项目(2005B34201021)

In vitro susceptibility of genital Chlamychia trachomatis to several antimicrobial agents

HAN Jian-de, CHEN Mu-kai, LIAO Qi-man, WANG Li-hong, LING Hong-zhong, TU Yu-ying   

  1. Department of Dermatology, First AFfiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China
  • Received:2006-02-22 Online:2006-04-15 Published:2006-04-15

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摘要： 目的 检测和对比数年来泌尿生殖道沙眼衣原体(Ct)临床分离株对几种常用抗菌药物的敏感性.方法 分别选用1997年、1999年、2001年、2003年和2005年临床泌尿生殖道Ct分离株,用McCoy细胞培养法鉴定,检测7种药物抗Ct体外抗菌活性,测定其最小抑菌浓度(MIC)和最小杀菌浓度(MBC).结果 1997-2005年,几种常用药物MIC90、MBC90均有不同程度升高,尤其是左氧氟沙星升高最为明显,MIC90由0.063 mg/L升高至1 mg/L,MBC90由0.063 mg/L升高至4 mg/L,2005年Ct临床分离株,克拉霉素、司帕沙星和莫西沙星杀菌浓度均较低,其MBC90分别为:0.25 mg/L、0.125 mg/L和0.063 mg/L.结论 Ct对几种常用抗菌药物的体外敏感性降低;目前Ct临床分离株对克拉霉素、司帕沙星和新药莫西沙星有较好敏感性.

关键词: 衣原体,沙眼, 微生物敏感性试验, 大环内酯类, 米诺环素, 喹诺酮类

Abstract: Objective To detect and compare the in vitro activities of several antimicrobial agents against isolates of Chlamydia trachomatis (Ct) cultured from the genitalia in the past several years.Methods Strains of Ct cultured in 1997,1999,2001,2003 and 2005 were selected.The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of 7 antibacterial agents,including erythromycin,minocycline,azithromycin,clarithromycin,levo-ofloxacin,sparfloxacin and moxifloxacin,against these strains were assessed in McCoy cell culture.Results The MIC90 and MBC90 of the these drugs increased from 1997 to 2005,especially those of levofloxacin,which increased from 0.063 mg/L to 1 mg/L and from 0.063 mg/L to 4 mg/L,respectively.The MIC90 and MBC90 of clarithromycin,sparfloxacin and moxifloxacin against clinical isolates of Ct in 2005 were relatively low,with the MBC90 of 0.25 mg/L,0.125 mg/L and 0.063 mg/L respectively.Conclusions In the past several years,the in vitro effectiveness of these antimicrobial agents against clinical Ct isolates have gradually decreased;clarithromycin,sparfloxacin and moxifloxacin showed good in vitro activity against the most recent isolates of genital Ct.

Key words: Chlamydia trachomatis, Microbial sensitivity tests, Macrolides, Minocycline, Quniolones