中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (9): 578-580.

• 论著 • 上一篇    下一篇

斑块状银屑病外周血单一核细胞p16基因甲基化状态的研究

季江, 崔盘根, 陈敏, 李遇梅, 李晶冰, 陈志强   

  1. 中国医学科学院中国协和医科大学皮肤病研究所, 南京210042
  • 收稿日期:2005-01-31 发布日期:2005-09-15

Study on Methylation Status of pl6 Gene in PBMC of Patients with Plaque Psoriasis

JI Jiang, CUI Pan-gen, CHEN Min, LI Yu-mei, LI Jing-bing, CHEN Zhi-qiang   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042, China
  • Received:2005-01-31 Published:2005-09-15

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摘要： 目的 探讨银屑病患者外周血单一核细胞p16基因甲基化状态。方法 采用甲基化特异的聚合酶链反应对34例斑块状银屑病患者和35例正常人外周血单一核细胞p16基因甲基化状态进行分析,银屑病的严重程度按PASI评分评估。结果 斑块状银屑病外周血单一核细胞p16基因甲基化率高于正常人,差异有统计学意义(P<0.05),但单一核细胞p16基因甲基化状态对疾病的严重程度和病程无明显影响(P>0.05)。结论 斑块状银屑病外周血单一核细胞p16基因甲基化率明显增高,在银屑病的发病机制中可能起某些作用。

关键词: 银屑病, 基因, p16, 甲基化

Abstract: Objective To study the methylation status of p16 gene in peripheral blood mononuclear cell (PBMC) of patients with plaque psoriasis, and to study its significance in the pathogenesis of the disease. Methods Thirty-four cases of psoriatic patients and 35 healthy controls were measured for the status of promoter methylation in p16 gene by methylation specific PCR (MSP). A region containing CpG site in p16 gene promoter was amplified by MSP. The severity of psoriasis was evaluated by PASI scores. Results The methylation rates of p16 gene in PBMC were significantly higher (P<0.05) in psoriatic patients than those in the controls, but it was unrelated to the severity and course of the disease. Conclusions Higher rates of hypermethylation of p16 gene are found in PBMC of the patients with plaque psoriasis, suggesting that p16 gene hypermethylation of PBMC may be involved in the pathogenesis of psoriasis.

Key words: Psoriasis, Genes, p16, Methylation