中华皮肤科杂志 ›› 2020, Vol. 53 ›› Issue (12): 1033-1036.doi: 10.35541/cjd.20190603

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皮肤科领域单细胞转录组测序研究进展

谷晶晶1    邓成成2    杨斌3   

  1. 1南方医科大学第一临床医学院皮肤科,广州  510000;2南方医科大学皮肤病医院实验研究中心,广州  510000;3南方医科大学皮肤病医院皮肤科,广州  510000 
  • 收稿日期:2019-05-22 修回日期:2020-01-02 发布日期:2020-12-02
  • 通讯作者: 杨斌 E-mail:Yangbin101@hotmail.com

Single-cell RNA-sequencing in dermatology

Gu Jingjing1, Deng Chengcheng2, Yang Bin3   

  1. 1Department of Dermatology, The First College of Clinical Medicine, Southern Medical University, Guangzhou 510000, China; 2Department of Experimental Research, Dermatology Hospital of Southern Medical University, Guangzhou 510000, China; 3Department of Dermatology, Dermatology Hospital of Southern Medical University, Guangzhou 510000, China
  • Received:2019-05-22 Revised:2020-01-02 Published:2020-12-02
  • Contact: Yang Bin E-mail:Yangbin101@hotmail.com

摘要: 单细胞RNA测序(scRNA-seq)是在单个细胞水平上对其含有的转录组信息进行测序分析,用于发现新的细胞亚型,揭示细胞异质性,监视疾病的动态发展过程,最终提高对组织、器官和生物复杂性的理解。目前scRNA-seq已广泛应用于肿瘤和胚胎发育等领域,在皮肤科领域的应用也陆续增多。本文将简要介绍scRNA-seq的技术流程,并重点阐述其在皮肤科领域的研究进展。

关键词: 序列分析, RNA, 转录组, 单细胞分析, 遗传异质性, 银屑病, 白癜风, 黑色素瘤, 淋巴瘤, T细胞, 皮肤, 肿瘤, 鳞状细胞

Abstract: Single-cell RNA sequencing (scRNA-seq) is a technique to analyze the transcriptome sequence at the single cell level. It can be used to discover new cell subtypes, reveal heterogeneity between cells, track the progression of diseases, and eventually improve the understanding of complexity of tissues, organs and organisms. At present, scRNA-seq has been widely applied in the fields of tumor and embryonic development, and its application in dermatology has shown a gradual growth. This review briefly introduces technical procedures of scRNA-seq, and mainly elaborates relevant progress in dermatology.

Key words: Sequence analysis, RNA, Transcriptome, Single-cell analysis, Genetic heterogeneity, Psoriasis, Vitiligo, Melanoma, Lymphoma, T-cell, cutaneous, Neoplasms, squamous cell

引用本文

谷晶晶 邓成成 杨斌. 皮肤科领域单细胞转录组测序研究进展[J]. 中华皮肤科杂志, 2020,53(12):1033-1036. doi:10.35541/cjd.20190603

Gu Jingjing, Deng Chengcheng, Yang Bin. Single-cell RNA-sequencing in dermatology[J]. Chinese Journal of Dermatology, 2020, 53(12): 1033-1036.doi:10.35541/cjd.20190603