中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (7): 432-434.

• 论著 • 上一篇    下一篇

低剂量长波紫外线诱导培养的人皮肤黑素细胞适应性反应观察

刘仲荣1, 刘玉峰2, 张海龙2, 赵小东2, 孙林潮2, 卢涛2, 高天文2   

  1. 1. 广州军区总医院皮肤科 510010;
    2. 第四军医大学第一附属医院皮肤科
  • 收稿日期:2004-07-05 发布日期:2005-07-15
  • 通讯作者: 刘玉峰, E-mail:Liuyuf@fmmu.edu.cn E-mail:Liuyuf@fmmu.edu, cn
  • 基金资助:
    博士后基金资助课题(2002224)

Low Dose UVA Radiation Induced Adaptation Reaction in Human Cultured Skin Melanocytes

LIU Zhong-rong1, LIU Yu-feng2, ZHANG Hai-long2, ZHAO Xiao-dong2, SUN Lin-chao2, LU Tao2, GAO Tian-wen2   

  1. Department of Dermatology, First Affiliated Hospital of Fourth Military Medical University, Xi'an 710032, China
  • Received:2004-07-05 Published:2005-07-15

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摘要： 目的 探讨低剂量长波紫外线(UVA)诱导培养人皮肤黑素细胞适应性反应的程度及特点。方法 以具有致死作用的86.4J/cm2UVA照射经7.2J/cm2低剂量UVA单次或多次预照射的培养人皮肤黑素细胞.光镜、电镜观察细胞形态学变化,流式细胞仪检测细胞凋亡的比例,单细胞凝胶电泳检测DNA损伤的程度。结果 单次或多次7.2J/cm2UVA预照射处理后的培养皮肤黑素细胞使随后86.4J/cm2UVA照射诱导的形态学上的毒性反应减轻,细胞凋亡的比例下降,DNA链断裂减少及修复加快,与未经预处理86.4J/cm2UVA照射的相应细胞比较差异有统计学意义(P<0.01或P<0.05);单次7.2J/cm2UVA预照射诱导培养皮肤黑素细胞的适应性反应在预照射12h后消失,而当低剂量UVA预照射的累积剂量达到28.8J/cm2以上时,预照射的培养细胞即使是14d后对86.4J/cm2UVA照射仍有明显的防护作用。结论 低剂量UVA照射可诱导培养的皮肤黑素细胞出现对随后高剂量UVA照射的适应性反应,其效应滞留期及强度与低剂量UVA的累积剂量有关。

关键词: 紫外线, 黑素细胞, 细胞,培养的, 适应,生物学

Abstract: Objective To investigate the extent and features of adaptation reaction of melanocytes induced by low dose UVA radiation.Methods Cultured skin melanocytes were irradiated by a lethal dose of 86.4 J/cm2 UVA with or without pre-irradiation of single or repetitive low dose 7.2 J/cm2 UVA.Alterations of cellular morphology were observed by light and electron microscopy.Proportions of apoptosis were assayed by flow cytometry.The extent of DNA damage was determined by single cell gel electrophoresis (SCGE).Results In cultured skin melanocytes,pretreatment with single or repetitive irradiation of 7.2 J/cm2 UVA could alleviate toxic reaction of cellular morphology,decrease apoptosis ratio,reduce DNA chain breakages and enhance repair of DNA damage caused by subsequent 86.4 J/cm2 UVA irradiations,compared to those without pretreatment (P<0.01 or P<0.05).The adaptation reaction induced by a single pretreatment of 7.2 J/cm2 UVA disappeared 12 h after irradiation,however,the accumulated dosage of 28.8 J/cm2 by repeated low dose UVA pretreatments could prevent damage by 86.4 J/cm2 UVA irradiation even after 14 d.Conclusions Low dose U-VA irradiation could induce adaptation reaction which protects cultured skin melanocytes from damages by subsequent challenged dose of UVA.The period the adaptation reaction lasts and protective extents are clearly related to the accumulated dosage of repeated low dose UVA irradiations and intervals.

Key words: Ultraviolet rays, Melanocytes, Cells,cultured, Adaptation,biological